Title |
Crystallization and structure elucidation of GDSL esterase of Photobacterium sp. J15
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Published in |
International Journal of Biological Macromolecules, August 2018
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DOI | 10.1016/j.ijbiomac.2018.08.022 |
Pubmed ID | |
Authors |
Sharifah Nur Hidayah Syed Mazlan, Mohd Shukuri Mohamad Ali, Raja Noor Zaliha Raja Abd Rahman, Suriana Sabri, Mohd Anuar Jonet, Thean Chor Leow |
Abstract |
GDSL esterase J15 (EstJ15) is a member of Family II of lipolytic enzyme. The enzyme was further classified in subgroup SGNH hydrolase due to the presence of highly conserve motif, Ser-Gly-Asn-His in four conserved blocks I, II, III, and V, respectively. X-ray quality crystal of EstJ15 was obtained from optimized formulation containing 0.10 M ammonium sulphate, 0.15 M sodium cacodylate trihydrate pH 6.5, and 20% PEG 8000. The crystal structure of EstJ15 was solved at 1.38 Å with one molecule per asymmetric unit. The structure exhibits α/β hydrolase fold and shared low amino acid sequence identity of 23% with the passenger domain of the autotransporter EstA of Pseudomonas aeruginosa. The active site is located at the centre of the structure, formed a narrow tunnel that hinder long substrates to be catalysed which was proven by the protein-ligand docking analysis. This study facilitates the understanding of high substrate specificity of EstJ15 and provide insights on its catalytic mechanism. |
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