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De novo transcriptome analysis shows differential expression of genes in salivary glands of edible bird’s nest producing swiftlets

Overview of attention for article published in BMC Genomics, July 2017
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Title
De novo transcriptome analysis shows differential expression of genes in salivary glands of edible bird’s nest producing swiftlets
Published in
BMC Genomics, July 2017
DOI 10.1186/s12864-017-3861-9
Pubmed ID
Authors

Q. H. Looi, H. Amin, I. Aini, M. Zuki, A. R. Omar

Abstract

Edible bird's nest (EBN), produced from solidified saliva secretions of specific swiftlet species during the breeding season, is one of the most valuable animal by-products in the world. The composition and medicinal benefits of EBN have been extensively studied, however, genomic and transcriptomic studies of the salivary glands of these birds have not been conducted. The study described the transcriptomes of salivary glands from three swiftlet species (28 samples) generated by RNASeq. A total of 14,835 annotated genes and 428 unmapped genes were cataloged. The current study investigated the genes and pathways that are associated with the development of salivary gland and EBN composition. Differential expression and pathway enrichment analysis indicated that the expression of CREB3L2 and several signaling pathways involved in salivary gland development, namely, the EGFR, BMP, and MAPK signaling pathways, were up-regulated in swiftlets producing white EBN (Aerodramus fuciphagus) and black EBN (Aerodramus maximus) compared with non-EBN-producing swiftlets (Apus affinis). Furthermore, MGAT, an essential gene for the biosynthesis of N-acetylneuraminic acid (sialic acid), was highly expressed in both white- and black-nest swiftlets compared to non-EBN-producing swiftlets. Interspecies comparison between Aerodramus fuciphagus and Aerodramus maximus indicated that the genes involved in N-acetylneuraminic and fatty acid synthesis were up-regulated in Aerodramus fuciphagus, while alanine and aspartate synthesis pathways were up-regulated in Aerodramus maximus. Furthermore, gender-based analysis revealed that N-glycan trimming pathway was significantly up-regulated in male Aerodramus fuciphagus from its natural habitat (cave) compared to their female counterpart. Transcriptomic analysis of salivary glands of different swiftlet species reveal differential expressions of candidate genes that are involved in salivary gland development and in the biosynthesis of various bioactive compounds found in EBN.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 36 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 8 22%
Student > Ph. D. Student 5 14%
Researcher 5 14%
Student > Doctoral Student 2 6%
Student > Master 1 3%
Other 3 8%
Unknown 12 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 31%
Biochemistry, Genetics and Molecular Biology 3 8%
Computer Science 2 6%
Engineering 2 6%
Medicine and Dentistry 2 6%
Other 4 11%
Unknown 12 33%