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Characterization of canine coronavirus spread among domestic dogs in Vietnam

Overview of attention for article published in Journal of Veterinary Medical Science, November 2016
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Title
Characterization of canine coronavirus spread among domestic dogs in Vietnam
Published in
Journal of Veterinary Medical Science, November 2016
DOI 10.1292/jvms.16-0538
Pubmed ID
Authors

Dung van NGUYEN, Yukata TERADA, Shohei MINAMI, Kenzo YONEMITSU, Nao NAGATA, Thanh Dinh Ha LE, Ryusei KUWATA, Hiroshi SHIMODA, Ken MAEDA

Abstract

Canine coronavirus (CCoV) is an important pathogen that causes enteritis in dogs, but there is no information on CCoV infection in Vietnam. To examine the prevalence of CCoV infection among Vietnamese dogs, 201 serum samples were analyzed by virus-neutralization (VN) test. The results showed that antibody against CCoV-II was present in 87 dogs (43.3%). To detect genes of CCoV, fecal samples collected from 30 diarrheic and 50 healthy dogs were examinated by RT-PCR, confirming that 2 diarrheic dogs and 5 healthy dogs were positive for CCoV. Nucleotide sequences of N-terminal region of spike (S) gene indicated that CCoV strains were divided into two subgenotypes, CCoV-IIa and -IIb, respectively. Furthemore, we succeeded in isolating CCoV/dog/HCM47/2015, the isolate was plaque-purified three times, and 3'-terminal one-third of the genome was analyzed. Interestingly, the plaque-purified virus had a large deletion in ORF3abc and E genes (1,165 nt), and a short deletion in ORF7b gene (60 nt), suggesting that these regions are not necessary for in vitro replication of CCoV. Next, the antigenicity between the isolated CCoV-IIb and the other CCoV-IIa was compared by VN test, revealing that antigenicty of the isolated CCoV is equal or higher than that of the other CCoV. In summary, two subgenotypes of CCoV-II are spreading among Vietnamese dogs. The isolated virus with a large deletion after in vitro passage may be useful for the development of vaccine, owing to its antigenicity and efficient viral growth in vitro.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 19%
Student > Ph. D. Student 6 19%
Student > Master 4 13%
Student > Bachelor 3 9%
Student > Doctoral Student 1 3%
Other 5 16%
Unknown 7 22%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 9 28%
Biochemistry, Genetics and Molecular Biology 4 13%
Immunology and Microbiology 3 9%
Medicine and Dentistry 2 6%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Other 6 19%
Unknown 7 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 November 2016.
All research outputs
#22,756,649
of 25,371,288 outputs
Outputs from Journal of Veterinary Medical Science
#2,951
of 3,546 outputs
Outputs of similar age
#274,108
of 313,244 outputs
Outputs of similar age from Journal of Veterinary Medical Science
#33
of 40 outputs
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So far Altmetric has tracked 3,546 research outputs from this source. They receive a mean Attention Score of 2.5. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 40 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.