The data shown below were collected from the profiles of 102 X users who shared this research output. Click here to find out more about how the information was compiled.
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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Express barcoding with NextGenPCR and MinION for species‐level sorting of ecological samples
|
|---|---|
| Published in |
Molecular Ecology Resources, January 2024
|
| DOI | 10.1111/1755-0998.13922 |
| Pubmed ID | |
| Authors |
Cristina Vasilita, Vivian Feng, Aslak Kappel Hansen, Emily Hartop, Amrita Srivathsan, Robin Struijk, Rudolf Meier |
| Abstract |
The use of DNA barcoding is well established for specimen identification and large-scale biodiversity discovery, but remains underutilized for time-sensitive applications such as rapid species discovery in field stations, identifying pests, citizen science projects, and authenticating food. The main reason is that existing express barcoding workflows are either too expensive or can only be used in very well-equipped laboratories by highly-trained staff. We here show an alternative workflow combining rapid DNA extraction with HotSHOT, amplicon production with NextGenPCR thermocyclers, and sequencing with low-cost MinION sequencers. We demonstrate the power of the approach by generating 250 barcodes for 285 specimens within 6 h including specimen identification through BLAST. The workflow required only the following major equipment that easily fits onto a lab bench: Thermocycler, NextGenPCR, microplate sealer, Qubit, and MinION. Based on our results, we argue that simplified barcoding workflows for species-level sorting are now faster, more accurate, and sufficiently cost-effective to replace traditional morpho-species sorting in many projects. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 4 | 4% |
| United Kingdom | 2 | 2% |
| France | 2 | 2% |
| Australia | 1 | <1% |
| Poland | 1 | <1% |
| India | 1 | <1% |
| Finland | 1 | <1% |
| Bolivia, Plurinational State of | 1 | <1% |
| Unknown | 89 | 87% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 50 | 49% |
| Members of the public | 48 | 47% |
| Science communicators (journalists, bloggers, editors) | 4 | 4% |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 24% |
| Student > Ph. D. Student | 4 | 16% |
| Student > Bachelor | 2 | 8% |
| Student > Master | 2 | 8% |
| Professor | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 9 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 40% |
| Biochemistry, Genetics and Molecular Biology | 3 | 12% |
| Earth and Planetary Sciences | 2 | 8% |
| Environmental Science | 1 | 4% |
| Engineering | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 8 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 59. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 February 2024.
All research outputs
#766,517
of 26,547,438 outputs
Outputs from Molecular Ecology Resources
#43
of 2,001 outputs
Outputs of similar age
#13,042
of 385,520 outputs
Outputs of similar age from Molecular Ecology Resources
#1
of 34 outputs
Altmetric has tracked 26,547,438 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,001 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.8. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 385,520 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 34 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.