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A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells

Overview of attention for article published in Scientific Reports, January 2014
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (96th percentile)
  • High Attention Score compared to outputs of the same age and source (90th percentile)

Mentioned by

blogs
1 blog
twitter
14 X users
patent
13 patents

Citations

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540 Dimensions

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571 Mendeley
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Title
A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells
Published in
Scientific Reports, January 2014
DOI 10.1038/srep03594
Pubmed ID
Authors

Masato Nakagawa, Yukimasa Taniguchi, Sho Senda, Nanako Takizawa, Tomoko Ichisaka, Kanako Asano, Asuka Morizane, Daisuke Doi, Jun Takahashi, Masatoshi Nishizawa, Yoshinori Yoshida, Taro Toyoda, Kenji Osafune, Kiyotoshi Sekiguchi, Shinya Yamanaka

Abstract

In order to apply human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) to regenerative medicine, the cells should be produced under restricted conditions conforming to GMP guidelines. Since the conventional culture system has some issues that need to be addressed to achieve this goal, we developed a novel culture system. We found that recombinant laminin-511 E8 fragments are useful matrices for maintaining hESCs and hiPSCs when used in combination with a completely xeno-free (Xf) medium, StemFit™. Using this system, hESCs and hiPSCs can be easily and stably passaged by dissociating the cells into single cells for long periods, without any karyotype abnormalities. Human iPSCs could be generated under feeder-free (Ff) and Xf culture systems from human primary fibroblasts and blood cells, and they possessed differentiation abilities. These results indicate that hiPSCs can be generated and maintained under this novel Ff and Xf culture system.

X Demographics

X Demographics

The data shown below were collected from the profiles of 14 X users who shared this research output. Click here to find out more about how the information was compiled.
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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 571 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 4 <1%
Japan 2 <1%
Italy 2 <1%
France 1 <1%
Australia 1 <1%
Germany 1 <1%
India 1 <1%
Turkey 1 <1%
Finland 1 <1%
Other 1 <1%
Unknown 556 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 110 19%
Student > Ph. D. Student 107 19%
Student > Master 65 11%
Student > Bachelor 36 6%
Student > Doctoral Student 32 6%
Other 94 16%
Unknown 127 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 143 25%
Biochemistry, Genetics and Molecular Biology 111 19%
Medicine and Dentistry 68 12%
Neuroscience 33 6%
Engineering 22 4%
Other 49 9%
Unknown 145 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 34. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 August 2024.
All research outputs
#1,225,281
of 26,090,071 outputs
Outputs from Scientific Reports
#12,236
of 144,809 outputs
Outputs of similar age
#12,887
of 322,020 outputs
Outputs of similar age from Scientific Reports
#61
of 668 outputs
Altmetric has tracked 26,090,071 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 144,809 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.9. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 322,020 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 668 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.