Title |
Tbx18-positive cells differentiated from murine ES cells serve as proepicardial progenitors to give rise to vascular smooth muscle cells and fibroblasts
|
---|---|
Published in |
Biomedical Research (0970-938X), January 2017
|
DOI | 10.2220/biomedres.38.229 |
Pubmed ID | |
Authors |
Nobuhito Ikeda, Natsumi Nakazawa, Yasutaka Kurata, Hisako Yaura, Fikri Taufiq, Hiroyuki Minato, Akio Yoshida, Haruaki Ninomiya, Yuji Nakayama, Masanari Kuwabara, Yasuaki Shirayoshi, Ichiro Hisatome |
Abstract |
Proepicardium (PE) cells generate cardiac fibroblasts, smooth muscle cells (SMCs) and endothelial cells that form coronary arteries. T-box18 (Tbx18) is a well-known marker of PE cells and epicardium. We examined whether Tbx18-positive cells differentiated from murine embryonic stem (ES) cells serve as PE progenitors to give rise to vascular SMCs and fibroblasts. To collect Tbx18-positive cells, we established Tbx18-EGFP knock-in mouse ES cells using the CRISPR/Cas9 system. We harvested the Tbx18-EGFP-positive cells on day 8, 10 and 14 after the initiation of differentiation; Tbx18 mRNA was enriched on day 8 to 14 and Snai2 mRNA was enriched on day 8 and 10, indicating successful collection of Tbx18-positive cells. Tbx18-EGFP-positive cells expressed the PE marker WT1 on day 8 and 10. They also expressed the SMC marker Acta2 and fibroblast markers Thy1 and Fsp1 on day 8 to 14, but did not express the endothelial cell marker PECAM or the cardiac cell marker CD166 or Myh7. In conclusion, Tbx18-positive cells represent a part of PE cells in the initial phase of differentiation and subsequently include SMCs as well as fibroblasts. These results indicate that Tbx18-positive cells serve as a PE progenitor to supply a variety of cells that contribute to the formation of coronary arteries. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 30% |
Librarian | 1 | 10% |
Other | 1 | 10% |
Lecturer | 1 | 10% |
Student > Ph. D. Student | 1 | 10% |
Other | 2 | 20% |
Unknown | 1 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 40% |
Veterinary Science and Veterinary Medicine | 1 | 10% |
Agricultural and Biological Sciences | 1 | 10% |
Medicine and Dentistry | 1 | 10% |
Engineering | 1 | 10% |
Other | 0 | 0% |
Unknown | 2 | 20% |