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Characterization of Plasmodium falciparum glutamate dehydrogenase-soluble antigen

Overview of attention for article published in Brazilian Journal of Medical and Biological Research, September 1998
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Title
Characterization of Plasmodium falciparum glutamate dehydrogenase-soluble antigen
Published in
Brazilian Journal of Medical and Biological Research, September 1998
DOI 10.1590/s0100-879x1998000900008
Pubmed ID
Authors

A. Rodríguez-Acosta, N.G. Domínguez, I. Aguilar, M.E. Girón

Abstract

The major aim of this study was to characterize a soluble Plasmodium falciparum antigen from the plasma of malaria-infected humans and Plasmodium falciparum culture supernatants, using immunoabsorbent techniques and Western blotting. An Mr 60-kDa protein was isolated from the plasma of patients with Plasmodium falciparum malaria by affinity chromatography using rabbit anti-Proteus spp GDH(NADP+) serum as ligand. This protein, present in plasma of patients with acute Plasmodium falciparum infection, in Plasmodium falciparum culture supernatants, and in immune complexes, was tested with Plasmodium falciparum malaria hyperimmune serum from patients living in hyperendemic areas and rabbit anti-Proteus spp GDH(NADP+) serum prepared in the laboratory. In this report, we describe the results of a study showing that parasite GDH(NADP+) can be used to detect the presence of Plasmodium falciparum. It appears that this technique permits the chromatographic detection of a Plasmodium falciparum excretion antigen that may be used in the production of monoclonal antibodies to improve immunodiagnostic assays for the detection of antigenemia, and opens the possibility of its use as a non-microscopic screening method.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 27 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 22%
Student > Ph. D. Student 6 22%
Student > Bachelor 3 11%
Student > Postgraduate 3 11%
Student > Doctoral Student 2 7%
Other 2 7%
Unknown 5 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 22%
Biochemistry, Genetics and Molecular Biology 5 19%
Medicine and Dentistry 2 7%
Engineering 2 7%
Chemistry 2 7%
Other 3 11%
Unknown 7 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 September 2021.
All research outputs
#8,534,528
of 25,373,627 outputs
Outputs from Brazilian Journal of Medical and Biological Research
#294
of 1,254 outputs
Outputs of similar age
#10,217
of 31,966 outputs
Outputs of similar age from Brazilian Journal of Medical and Biological Research
#17
of 54 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,254 research outputs from this source. They receive a mean Attention Score of 4.9. This one has gotten more attention than average, scoring higher than 50% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 31,966 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 9th percentile – i.e., 9% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 54 others from the same source and published within six weeks on either side of this one. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.