Chapter title |
In Vivo Analysis of Dendritic Cell Clonality.
|
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Chapter number | 4 |
Book title |
Dendritic Cells
|
Published in |
Methods in molecular biology, January 2023
|
DOI | 10.1007/978-1-0716-2938-3_4 |
Pubmed ID | |
Book ISBNs |
978-1-07-162937-6, 978-1-07-162938-3
|
Authors |
Cabeza-Cabrerizo, Mar |
Abstract |
Confocal fluorescence microscopy is commonly used for the analysis of tissue architecture and cell distribution (Paddock, Confocal microscopy: methods and protocols. Methods in molecular biology. Humana Press, New York, pp 1-388, 2013). When combined with multicolor fate mapping of cell precursors, it allows for analysis of single-color cell clusters, which in turn informs on the clonal relationship of cells in tissues (Snippert et al, Cell 143:134-144. https://doi.org/10.1016/j.cell.2010.09.016 , 2010). In this chapter, I describe a multicolor fate mapping mouse model and microscopy technique to trace the progeny of conventional dendritic cell (cDC, (Cabeza-Cabrerizo et al, Annu Rev Immunol 39:131. https://doi.org/10.1146/annurev-immunol-061020-053707 , 2021)) progenitors in different tissues and analyze cDC clonality. The chapter is focused on imaging methods rather than image analysis, although the software used to quantify cluster formation is also introduced. |
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