Systems Biology
Springer US
Chapter title |
Optical Estimation of Bioelectric Patterns in Living Embryos.
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Chapter number | 6 |
Book title |
Systems Biology
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Published in |
Methods in molecular biology, January 2024
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DOI | 10.1007/978-1-0716-3577-3_6 |
Pubmed ID | |
Book ISBNs |
978-1-07-163576-6, 978-1-07-163577-3
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Authors |
McMillen, Patrick, Levin, Michael |
Abstract |
Fluorescent lifetime imaging (FLIM) is a powerful tool for visualizing physiological parameters in vivo. We present here a 3-dye strategy for mapping bioelectric patterns in living Xenopus laevis embryos leveraging the quantitative power of fluorescent lifetime imaging. We discuss a general strategy for disentangling physiological artifacts from true bioelectric signals, a method for dye delivery via transcardial injection, and how to visualize and interpret the fluorescent lifetime of the dyes in vivo. |
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Members of the public | 7 | 50% |
Scientists | 6 | 43% |
Science communicators (journalists, bloggers, editors) | 1 | 7% |
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