Chapter title |
Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP)
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Chapter number | 20 |
Book title |
Nuclear Reprogramming
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Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-1594-1_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1593-4, 978-1-4939-1594-1
|
Authors |
Hélène Kiefer, Kiefer, Hélène |
Editors |
Nathalie Beaujean, Hélène Jammes, Alice Jouneau |
Abstract |
Methylated DNA immunoprecipitation (MeDIP), when coupled to high-throughput sequencing or microarray hybridization, allows for the identification of methylated loci at a genome-wide scale. Genomic regions affected by incomplete reprogramming after nuclear transfer can potentially be delineated by comparing the MeDIP profiles of bovine clones and non-clones. This chapter presents a MeDIP protocol largely inspired from Mohn and colleagues (Mohn et al., Methods Mol Biol 507:55-64, 2009), with PCR primers specific for cattle, and when possible, overviews of experimental designs adapted to the comparison between clones and non-clones. |
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