Chapter title |
Identification of Neural Stem Cell Biomarkers by Isobaric Tagging for Relative and Absolute Quantitation (iTRAQ) Mass Spectrometry
|
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Chapter number | 34 |
Book title |
Investigations of Early Nutrition Effects on Long-Term Health
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7614-0_34 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7613-3, 978-1-4939-7614-0
|
Authors |
Paul C. Guest |
Abstract |
This chapter describes a proteomic analysis of neural progenitor cells using isobaric tagging for relative and absolute quantitation (iTRAQ) mass spectrometry. A detailed procedure is described for the isolation, proliferation, and differentiation of these cells, including a comparative iTRAQ mass spectrometry analysis of the precursor and differentiated states. In total, there were changes in the levels of 55 proteins, many of which are not resolved easily by other proteomic methods. Therefore, this method should be useful for the identification of important regulatory molecules in the study of other precursor cells involved in neuronal or metabolic regulation in nutritional programming diseases. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 3 | 100% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 2 | 67% |
Biochemistry, Genetics and Molecular Biology | 1 | 33% |