Chapter title |
Mass Spectrometry Profiling of Pituitary Glands
|
---|---|
Chapter number | 31 |
Book title |
Investigations of Early Nutrition Effects on Long-Term Health
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7614-0_31 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7613-3, 978-1-4939-7614-0
|
Authors |
Divya Krishnamurthy, Hassan Rahmoune, Paul C. Guest, Krishnamurthy, Divya, Rahmoune, Hassan, Guest, Paul C. |
Abstract |
Many chronic diseases are associated with hypothalamic-pituitary-adrenal axis dysfunction. Therefore, proteomic profiling of the pituitary gland has potential to uncover new information on the underlying pathways affected in these conditions. This could lead to identification of new biomarkers or drug targets for development of novel therapeutics. Here we present a protocol for preparation of pituitary protein extracts and analysis of the major hormones and accessory proteins using liquid chromatography tandem mass spectrometry (LC-MS/MS). The same methods can be applied in the study of other tissues of the diffuse neuroendocrine system. |
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