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Mendeley readers
Chapter title |
Detection of Thermospermine and Spermine by HPLC in Plants
|
---|---|
Chapter number | 6 |
Book title |
Polyamines
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7398-9_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7397-2, 978-1-4939-7398-9
|
Authors |
Taku Takahashi, Ayaka Takano, Jun-Ichi Kakehi |
Abstract |
Thermospermine, a structural isomer of spermine, is widely spread in the plant kingdom and has recently been shown to play a key role in the repression of xylem differentiation in vascular plants. However, a standard high-performance liquid chromatography (HPLC) protocol for detecting polyamines as their dansyl derivative cannot distinguish themospermine from spermine. These isomers become separated from each other after benzoylation. In this chapter, we describe a simple protocol for extraction, benzoylation, and HPLC detection of thermospermine and spermine with other polyamines from plant material. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 55% |
Student > Bachelor | 1 | 9% |
Professor | 1 | 9% |
Student > Doctoral Student | 1 | 9% |
Unknown | 2 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 36% |
Biochemistry, Genetics and Molecular Biology | 3 | 27% |
Unspecified | 1 | 9% |
Unknown | 3 | 27% |