Chapter title |
DNA methylation analysis of germ cells by using bisulfite-based sequencing methods.
|
---|---|
Chapter number | 17 |
Book title |
Germline Development
|
Published in |
Methods in molecular biology, December 2011
|
DOI | 10.1007/978-1-61779-436-0_17 |
Pubmed ID | |
Book ISBNs |
978-1-61779-435-3, 978-1-61779-436-0
|
Authors |
Kobayashi H, Kono T, Hisato Kobayashi, Tomohiro Kono, Kobayashi, Hisato, Kono, Tomohiro |
Abstract |
Dynamic changes in DNA methylation at the gene-specific and genome-wide level occur during mammalian germ-cell development. However, the details of how the methylation profiles change remain largely unknown. Bisulfite sequencing analysis is a powerful technique to determine the methylation status of DNA at individual cytosine-guanine dinucleotide (CpG) sites and requires only a small amount of DNA for analysis. Here, we introduce two methods for bisulfite-based DNA methylation analyses using small samples such as germ cells: bisulfite Sanger sequencing at a specific locus and high-throughput bisulfite sequencing at the whole genome level. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
Australia | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 54% |
Professor > Associate Professor | 3 | 23% |
Student > Ph. D. Student | 1 | 8% |
Student > Postgraduate | 1 | 8% |
Unknown | 1 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 8 | 62% |
Biochemistry, Genetics and Molecular Biology | 2 | 15% |
Nursing and Health Professions | 1 | 8% |
Social Sciences | 1 | 8% |
Unknown | 1 | 8% |