Chapter title |
Continuous High-Throughput Colorimetric Assays for α -Transaminases
|
---|---|
Chapter number | 13 |
Book title |
Protein Engineering
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7366-8_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7364-4, 978-1-4939-7366-8
|
Authors |
Egon Heuson, Jean-Louis Petit, Franck Charmantray, Véronique de Bérardinis, Thierry Gefflaut, Heuson, Egon, Petit, Jean-Louis, Charmantray, Franck, Bérardinis, Véronique de, Gefflaut, Thierry |
Abstract |
Transaminases are efficient tools for the stereoselective conversion of prochiral ketones into valuable chiral amines. Notably, the diversity of naturally occurring α-transaminases offers access to a wide range of L- and D-α-amino acids. We describe here two continuous colorimetric assays for the quantification of transamination activities between a keto acid and a standard donor substrate (L- or D-Glutamic acid or cysteine sulfinic acid). These assays are helpful for kinetic studies as well as for high-throughput screening of enzyme collections. |
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