Chapter title |
Quantifying Re-association of a Facultative Endohyphal Bacterium with a Filamentous Fungus
|
---|---|
Chapter number | 1 |
Book title |
Plant Pathogenic Fungi and Oomycetes
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8724-5_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8723-8, 978-1-4939-8724-5
|
Authors |
David A. Baltrus, Joseph Spraker, A. Elizabeth Arnold, Baltrus, David A., Spraker, Joseph, Arnold, A. Elizabeth |
Abstract |
We present here a method to quantify reassociation between facultative endohyphal bacteria and filamentous fungal hosts. Our method takes advantage of the capabilities of fungal cell walls to selectively protect internal bacteria from gentamicin treatment, an assay adapted from studies of internalized bacterial pathogens in cell culture. We report the efficacy of gentamicin to kill planktonic bacteria treated during fungal coculture, and also describe and characterize a sampling scheme to recover and quantify culturable bacteria from the growing edge of fungal mycelium in vitro. This assay enables qualitative and quantitative tests of reassociation capabilities for facultative endohyphal bacteria with host fungi and provides a means to investigate the genetic basis for these associations in a repeatable way. |
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Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Postgraduate | 2 | 8% |
Researcher | 1 | 4% |
Student > Ph. D. Student | 1 | 4% |
Unknown | 22 | 85% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 3 | 12% |
Environmental Science | 1 | 4% |
Unknown | 22 | 85% |