Title |
VEGFR2 Trafficking, Signaling and Proteolysis is Regulated by the Ubiquitin Isopeptidase USP8
|
---|---|
Published in |
Traffic, December 2015
|
DOI | 10.1111/tra.12341 |
Pubmed ID | |
Authors |
Gina A. Smith, Gareth W. Fearnley, Izma Abdul‐Zani, Stephen B. Wheatcroft, Darren C. Tomlinson, Michael A. Harrison, Sreenivasan Ponnambalam |
Abstract |
Vascular endothelial growth factor A (VEGF-A) regulates many aspects of vascular function. VEGF-A binding to vascular endothelial growth factor receptor 2 (VEGFR2) stimulates endothelial signal transduction and regulates multiple cellular responses. Activated VEGFR2 undergoes ubiquitination but the enzymes that regulate this post-translational modification are unclear. In this study, the de-ubiquitinating enzyme, USP8, is shown to regulate VEGFR2 trafficking, de-ubiquitination, proteolysis and signal transduction. USP8-depleted endothelial cells displayed altered VEGFR2 ubiquitination and production of a unique VEGFR2 extracellular domain proteolytic fragment caused by VEGFR2 accumulation in the endosome-lysosome system. In addition, perturbed VEGFR2 trafficking impaired VEGF-A-stimulated signal transduction in USP8-depleted cells. Thus, regulation of VEGFR2 ubiquitination and de-ubiquitination has important consequences for the endothelial cell response and vascular physiology. |
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Mendeley readers
Geographical breakdown
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Spain | 1 | 2% |
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Demographic breakdown
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Researcher | 8 | 16% |
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Student > Master | 5 | 10% |
Professor > Associate Professor | 3 | 6% |
Other | 8 | 16% |
Unknown | 9 | 18% |
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Chemistry | 2 | 4% |
Other | 3 | 6% |
Unknown | 11 | 22% |