Christiane H. Antoni, Yvonne McDuffie, Jochen Bauer, Jonathan P. Sleeman, Heike Boehm
Abstract
Controlled activation of lymphangiogenesis through functional biomaterials represents a promising approach to support wound healing after surgical procedures, yet remains a challenge. In a synthetic biological approach, we therefore set out to mimic the basal microenvironment of human primary dermal lymphatic endothelial cells (LECs) during lymphangiogenesis. As the extracellular matrix component hyaluronan (HA) regulates lymphangiogenesis, we designed a bifunctional surface in which adhesive peptide ligands and short HA oligosaccharides (sHA) tethered to nanoparticles are copresented to the basal side of LECs in a controlled, concentration-dependent manner. Exposure of LECs to sHA in solution to mimic luminal stimulation of the cells did not result in modified metabolic activity. However, LECs grown on the bifunctional adhesive surfaces showed a biphasic change in metabolic activity, with increased metabolic activity being observed in response to increasing nanoparticle densities up to a maximum of 540 particles/μm2. Thus, interfaces that concomitantly present adhesive ligands and sHA can stimulate LEC metabolism and might be able to trigger lymphangiogenesis.
Pharmacology, Toxicology and Pharmaceutical Science
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13%
Agricultural and Biological Sciences
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13%
Other
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0%
Unknown
1
13%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 March 2018.
All research outputs
#18,591,506
of 23,028,364 outputs
Outputs from Frontiers in Bioengineering and Biotechnology
#3,442
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Outputs of similar age
#257,517
of 331,443 outputs
Outputs of similar age from Frontiers in Bioengineering and Biotechnology
#36
of 48 outputs
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