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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Construction of a New Phage Integration Vector pFIV-Val for Use in Different Francisella Species
|
|---|---|
| Published in |
Frontiers in Cellular and Infection Microbiology, March 2018
|
| DOI | 10.3389/fcimb.2018.00075 |
| Pubmed ID | |
| Authors |
Hana Tlapák, Kristin Köppen, Kerstin Rydzewski, Roland Grunow, Klaus Heuner |
| Abstract |
We recently identified and described a putative prophage on the genomic island FhaGI-1 located within the genome of Francisella hispaniensis AS02-814 (F. tularensis subsp. novicida-like 3523). In this study, we constructed two variants of a Francisella phage integration vector, called pFIV1-Val and pFIV2-Val (Francisella Integration Vector-tRNAVal-specific), using the attL/R-sites and the site-specific integrase (FN3523_1033) of FhaGI-1, a chloramphenicol resistance cassette and a sacB gene for counter selection of transformants against the vector backbone. We inserted the respective sites and genes into vector pUC57-Kana to allow for propagation in Escherichia coli. The constructs generated a circular episomal form in E. coli which could be used to transform Francisella spp. where FIV-Val stably integrated site specifically into the tRNAVal gene of the genome, whereas pUC57-Kana is lost due to counter selection. Functionality of the new vector was demonstrated by the successfully complementation of a Francisella mutant strain. The vectors were stable in vitro and during host-cell infection without selective pressure. Thus, the vectors can be applied as a further genetic tool in Francisella research, expanding the present genetic tools by an integrative element. This new element is suitable to perform long-term experiments with different Francisella species. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 50% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 25% |
| Student > Bachelor | 3 | 25% |
| Student > Master | 2 | 17% |
| Researcher | 1 | 8% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 4 | 33% |
| Computer Science | 1 | 8% |
| Veterinary Science and Veterinary Medicine | 1 | 8% |
| Sports and Recreations | 1 | 8% |
| Medicine and Dentistry | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 4 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 March 2019.
All research outputs
#15,494,712
of 23,026,672 outputs
Outputs from Frontiers in Cellular and Infection Microbiology
#3,630
of 6,512 outputs
Outputs of similar age
#213,366
of 333,763 outputs
Outputs of similar age from Frontiers in Cellular and Infection Microbiology
#64
of 109 outputs
Altmetric has tracked 23,026,672 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6,512 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.4. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 333,763 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 109 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.