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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Understanding the Virulence of Staphylococcus pseudintermedius: A Major Role of Pore-Forming Toxins
|
|---|---|
| Published in |
Frontiers in Cellular and Infection Microbiology, June 2018
|
| DOI | 10.3389/fcimb.2018.00221 |
| Pubmed ID | |
| Authors |
Yousef Maali, Cédric Badiou, Patrícia Martins-Simões, Elisabeth Hodille, Michele Bes, François Vandenesch, Gérard Lina, Alan Diot, Frederic Laurent, Sophie Trouillet-Assant |
| Abstract |
Staphylococcus pseudintermedius is responsible for severe and necrotizing infections in humans and dogs. Contrary to S. aureus, the pathophysiological mechanisms involved in this virulence are incompletely understood. We previously showed the intracellular cytotoxicity induced after internalization of S. pseudintermedius. Herein, we aimed to identify the virulence factors responsible for this cytotoxic activity. After addition of filtered S. pseudintermedius supernatants in culture cell media, MG63 cells, used as representative of non-professional phagocytic cells (NPPc), released a high level of LDH, indicating that the cytotoxicity was mainly mediated by secreted factors. Accordingly, we focused our attention on S. pseudintermedius toxins. In silico analysis found the presence of two PSMs (δ-toxin and PSMε) as well as Luk-I leukotoxin, the presence of which was confirmed by PCR in all clinical strains tested (n = 17). Recombinant Luk-I leukotoxin had no cytotoxic activity on NPPc but the ectopic expression of the CXCR2 receptor in U937 cells conferred cytotoxity to Luk-I. This is in agreement with the lack of Luk-I effect on NPPc and the previous report of Luk-I cytoxic activity on immune cells. Contrary to Luk-I, synthetic δ-toxin and PSMε had a strong cytotoxic activity on NPPc. The secretion of δ-toxin and PSMε at cytotoxic concentrations by S. pseudintermedius in culture supernatant was confirmed by HPLC-MS. In addition, the supplementation of such supernatants with human serum, known to inhibit PSM, induced a complete abolition of cytotoxicity which indicates that PSMs are the key players in the cytotoxic phenotype of NPPc. The results suggest that the severity of S. pseudintermedius infections is, at least in part, explained by a combined action of Luk-I that specifically targets immune cells expressing the CXCR2 receptor, and PSMs that disrupt cell membranes whatever the cell types. The present study strengthens the key role of PSMs in virulence of the different species belonging to Staphylococcus genus. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 5 | 45% |
| Portugal | 1 | 9% |
| United States | 1 | 9% |
| Unknown | 4 | 36% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 8 | 73% |
| Scientists | 3 | 27% |
Mendeley readers
The data shown below were compiled from readership statistics for 93 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 93 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 16 | 17% |
| Student > Master | 12 | 13% |
| Student > Ph. D. Student | 10 | 11% |
| Researcher | 9 | 10% |
| Lecturer | 5 | 5% |
| Other | 12 | 13% |
| Unknown | 29 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 23 | 25% |
| Immunology and Microbiology | 12 | 13% |
| Biochemistry, Genetics and Molecular Biology | 8 | 9% |
| Agricultural and Biological Sciences | 7 | 8% |
| Medicine and Dentistry | 5 | 5% |
| Other | 7 | 8% |
| Unknown | 31 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 78. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 October 2021.
All research outputs
#543,060
of 25,203,135 outputs
Outputs from Frontiers in Cellular and Infection Microbiology
#91
of 7,935 outputs
Outputs of similar age
#11,986
of 335,886 outputs
Outputs of similar age from Frontiers in Cellular and Infection Microbiology
#2
of 113 outputs
Altmetric has tracked 25,203,135 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 7,935 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.8. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 335,886 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 113 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.