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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Enhancement of RNA-directed DNA methylation of a transgene by simultaneously downregulating a ROS1 ortholog using a virus vector in Nicotiana benthamiana
|
|---|---|
| Published in |
Frontiers in Genetics, January 2013
|
| DOI | 10.3389/fgene.2013.00044 |
| Pubmed ID | |
| Authors |
Shungo Otagaki, Megumi Kasai, Chikara Masuta, Akira Kanazawa |
| Abstract |
Cytosine methylation can be induced by double-stranded RNAs through the RNA-directed DNA methylation (RdDM) pathway. A DNA glycosylase REPRESSOR OF SILENCING 1 (ROS1) participates in DNA demethylation in Arabidopsis and may possibly counteract RdDM. Here, we isolated an ortholog of ROS1 (NbROS1) from Nicotiana benthamiana and examined the antagonistic activity of NbROS1 against virus-induced RdDM by simultaneously inducing RdDM and NbROS1 knockdown using a vector based on Cucumber mosaic virus. Plants were inoculated with a virus that contained a portion of the Cauliflower mosaic virus 35S promoter, which induced RdDM of the promoter integrated in the plant genome and transcriptional silencing of the green fluorescent protein gene driven by the promoter. Plants were also inoculated with a virus that contained a portion of NbROS1, which induced downregulation of NbROS1. Simultaneous induction of RdDM and NbROS1 knockdown resulted in an increase in the level of cytosine methylation of the target promoter. These results provide evidence for the presence of antagonistic activity of NbROS1 against virus-induced RdDM and suggest that the simultaneous induction of promoter-targeting RdDM and NbROS1 knockdown by a virus vector is useful as a tool to enhance targeted DNA methylation. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 3% |
| United Kingdom | 1 | 3% |
| Australia | 1 | 3% |
| Unknown | 28 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 39% |
| Student > Ph. D. Student | 9 | 29% |
| Student > Master | 3 | 10% |
| Professor | 2 | 6% |
| Lecturer > Senior Lecturer | 1 | 3% |
| Other | 3 | 10% |
| Unknown | 1 | 3% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 26 | 84% |
| Biochemistry, Genetics and Molecular Biology | 3 | 10% |
| Unknown | 2 | 6% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2017.
All research outputs
#3,669,114
of 22,703,044 outputs
Outputs from Frontiers in Genetics
#1,105
of 11,755 outputs
Outputs of similar age
#38,900
of 280,707 outputs
Outputs of similar age from Frontiers in Genetics
#49
of 319 outputs
Altmetric has tracked 22,703,044 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 11,755 research outputs from this source. They receive a mean Attention Score of 3.7. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,707 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 319 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.