Angélique Lewies, Etresia Van Dyk, Johannes F. Wentzel, Pieter J. Pretorius
Abstract
The comet assay is a simple and cost effective technique, commonly used to analyze and quantify DNA damage in individual cells. The versatility of the comet assay allows introduction of various modifications to the basic technique. The difference in the methylation sensitivity of the isoschizomeric restriction enzymes HpaII and MspI are used to demonstrate the ability of the comet assay to measure the global DNA methylation level of individual cells when using cell cultures. In the experiments described here, a medium-throughput comet assay and methylation sensitive comet assay are combined to produce a methylation sensitive medium-throughput comet assay to measure changes in the global DNA methylation pattern in individual cells under various growth conditions.
Pharmacology, Toxicology and Pharmaceutical Science
3
7%
Medicine and Dentistry
1
2%
Other
2
5%
Unknown
11
26%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 July 2014.
All research outputs
#18,374,472
of 22,758,248 outputs
Outputs from Frontiers in Genetics
#7,009
of 11,758 outputs
Outputs of similar age
#161,539
of 225,738 outputs
Outputs of similar age from Frontiers in Genetics
#107
of 124 outputs
Altmetric has tracked 22,758,248 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 11,758 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
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We're also able to compare this research output to 124 others from the same source and published within six weeks on either side of this one. This one is in the 6th percentile – i.e., 6% of its contemporaries scored the same or lower than it.
