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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Immunodetection of NETs in Paraffin-Embedded Tissue
|
|---|---|
| Published in |
Frontiers in immunology, November 2016
|
| DOI | 10.3389/fimmu.2016.00513 |
| Pubmed ID | |
| Authors |
Volker Brinkmann, Ulrike Abu Abed, Christian Goosmann, Arturo Zychlinsky |
| Abstract |
The pathogenic potential of neutrophil extracellular traps (NETs) was recently described, and their detection in tissue could serve as a prognostic marker. NETs are delicate and filigree structures; hence good tissue preservation is essential for their detection. Indeed, analysis of paraffin-embedded tissue has proven superior to the study of cryo sections. Though, under favorable conditions, the presence of NETs can be detected in tissue sections stained with histological dyes, definitive identification of NETs needs the colocalization of immunofluorescent signals for both nuclear and granular (or cytoplasmic) NET components. We tested diverse antigen retrieval methods and various combinations of commercially available antibodies and present here staining protocols to detect NETs in human and murine tissue sections. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Iran, Islamic Republic of | 1 | 20% |
| Mexico | 1 | 20% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 3 | 60% |
| Members of the public | 2 | 40% |
Mendeley readers
The data shown below were compiled from readership statistics for 167 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Turkey | 1 | <1% |
| Unknown | 166 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 31 | 19% |
| Researcher | 28 | 17% |
| Student > Master | 19 | 11% |
| Student > Doctoral Student | 13 | 8% |
| Student > Bachelor | 9 | 5% |
| Other | 28 | 17% |
| Unknown | 39 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 35 | 21% |
| Biochemistry, Genetics and Molecular Biology | 27 | 16% |
| Medicine and Dentistry | 26 | 16% |
| Agricultural and Biological Sciences | 18 | 11% |
| Neuroscience | 4 | 2% |
| Other | 13 | 8% |
| Unknown | 44 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 November 2016.
All research outputs
#14,914,476
of 25,374,647 outputs
Outputs from Frontiers in immunology
#13,185
of 31,520 outputs
Outputs of similar age
#215,924
of 415,434 outputs
Outputs of similar age from Frontiers in immunology
#124
of 240 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 31,520 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.4. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 415,434 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 240 others from the same source and published within six weeks on either side of this one. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.