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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Under the Microscope: Single-Domain Antibodies for Live-Cell Imaging and Super-Resolution Microscopy
|
|---|---|
| Published in |
Frontiers in immunology, August 2017
|
| DOI | 10.3389/fimmu.2017.01030 |
| Pubmed ID | |
| Authors |
Bjoern Traenkle, Ulrich Rothbauer |
| Abstract |
Single-domain antibodies (sdAbs) have substantially expanded the possibilities of advanced cellular imaging such as live-cell or super-resolution microscopy to visualize cellular antigens and their dynamics. In addition to their unique properties including small size, high stability, and solubility in many environments, sdAbs can be efficiently functionalized according to the needs of the respective imaging approach. Genetically encoded intrabodies fused to fluorescent proteins (chromobodies) have become versatile tools to study dynamics of endogenous proteins in living cells. Additionally, sdAbs conjugated to organic dyes were shown to label cellular structures with high density and minimal fluorophore displacement making them highly attractive probes for super-resolution microscopy. Here, we review recent advances of the chromobody technology to visualize localization and dynamics of cellular targets and the application of chromobody-based cell models for compound screening. Acknowledging the emerging importance of super-resolution microscopy in cell biology, we further discuss advantages and challenges of sdAbs for this technology. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 201 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 201 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 37 | 18% |
| Student > Master | 27 | 13% |
| Researcher | 24 | 12% |
| Student > Bachelor | 20 | 10% |
| Student > Doctoral Student | 9 | 4% |
| Other | 22 | 11% |
| Unknown | 62 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 58 | 29% |
| Agricultural and Biological Sciences | 25 | 12% |
| Neuroscience | 12 | 6% |
| Chemistry | 10 | 5% |
| Immunology and Microbiology | 6 | 3% |
| Other | 24 | 12% |
| Unknown | 66 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 April 2023.
All research outputs
#7,359,319
of 25,382,440 outputs
Outputs from Frontiers in immunology
#8,558
of 31,537 outputs
Outputs of similar age
#108,177
of 324,941 outputs
Outputs of similar age from Frontiers in immunology
#147
of 445 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 31,537 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.4. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 324,941 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 445 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.