Title |
CD86 Expression by Monocytes Influences an Immunomodulatory Profile in Asymptomatic Patients with Chronic Chagas Disease
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Published in |
Frontiers in immunology, March 2018
|
DOI | 10.3389/fimmu.2018.00454 |
Pubmed ID | |
Authors |
Bruna F. Pinto, Nayara I. Medeiros, Andrea Teixeira-Carvalho, Silvana M. Eloi-Santos, Tereza C. M. Fontes-Cal, Débora A. Rocha, Walderez O. Dutra, Rodrigo Correa-Oliveira, Juliana A. S. Gomes |
Abstract |
In the chronic phase of Chagas disease, 60% of the patients develop the asymptomatic form known as indeterminate (IND). The remaining 30% of the patients develop a life-threatening form in which digestive and/or cardiac (CARD) alterations take place. The mechanisms underlying the development of severe forms of Chagas disease remain poorly understood. It is well known that interactions between immune cells such as monocytes and lymphocytes drive immune responses. Further, the co-stimulatory molecules CD80 and CD86 expressed by monocytes and subsets induce lymphocyte activation, thereby triggering cellular immune response. Here, we revealed, for the first time, the functional-phenotypic profile of monocytes subsets in Chagas disease. Using flow cytometry, we evaluated the effect ofin vitrostimulation withTrypanosoma cruziantigens on the expression of the co-stimulatory molecules CD80 and CD86 in different monocyte subsets of patients with IND and CARD clinical forms of Chagas disease. We also assessed the expression of toll-like receptor (TLR)-2, TLR-4, TLR-9, HLA-DR, IL-10, and IL-12 in the monocyte subsets and of CTLA-4 and CD28, ligands of CD80 and CD86, in T lymphocytes. CD86 expression in all monocyte subsets was higher in IND patients when compared with non-infected (NI) individuals. After stimulation withT. cruzi, these patients also showed a higher frequency of CD4+CTLA-4+T lymphocytes than NI individuals. We found an association between CD80 and CD28, and between CD86 and CTLA-4 expression, with a high frequency of regulatory T (Treg) cells in IND patients. We proposed that CD86 may be involved in immunoregulation by its association with CTLA-4 in asymptomatic patients. CD86 and CTLA-4 interaction may influence Treg activation, and this could represent a new strategy to control inflammation and tissue damage. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 65 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 11 | 17% |
Student > Bachelor | 7 | 11% |
Student > Doctoral Student | 7 | 11% |
Researcher | 6 | 9% |
Student > Master | 5 | 8% |
Other | 9 | 14% |
Unknown | 20 | 31% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 13 | 20% |
Immunology and Microbiology | 11 | 17% |
Agricultural and Biological Sciences | 8 | 12% |
Medicine and Dentistry | 5 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Other | 4 | 6% |
Unknown | 23 | 35% |