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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Effect of VH–VL Families in Pertuzumab and Trastuzumab Recombinant Production, Her2 and FcγIIA Binding
|
|---|---|
| Published in |
Frontiers in immunology, March 2018
|
| DOI | 10.3389/fimmu.2018.00469 |
| Pubmed ID | |
| Authors |
Wei-Li Ling, Wai-Heng Lua, Jun-Jie Poh, Joshua Yi Yeo, David Philip Lane, Samuel Ken-En Gan |
| Abstract |
Many therapeutic antibodies are humanized from animal sources. In the humanization process, complementarity determining region grafting is tedious and highly prone to failure. With seven known VH families, and up to six known κ VL families, there are choices aplenty. However, the functions of these families remain largely enigmatic. To study the role of these V-region families, we made 84 recombinant combinations of the various VH and VL family whole IgG1 variants of both Trastuzumab and Pertuzumab. We managed to purify 66 of these to investigate the biophysical characteristics: recombinant protein production, and both Her2 and FcγIIA binding. Our findings revealed combinations that showed improved recombinant antibody production and both antigen and receptor binding kinetics. These findings show the need to rethink antibodies as a whole protein, relooking of the functions of the antibody domains, and the need to include immunoglobulin receptor investigations for effective antibody therapeutics development. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Singapore | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 42 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 24% |
| Student > Bachelor | 8 | 19% |
| Student > Ph. D. Student | 4 | 10% |
| Student > Master | 3 | 7% |
| Professor | 1 | 2% |
| Other | 2 | 5% |
| Unknown | 14 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 26% |
| Agricultural and Biological Sciences | 5 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 10% |
| Immunology and Microbiology | 3 | 7% |
| Chemistry | 2 | 5% |
| Other | 3 | 7% |
| Unknown | 14 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 March 2020.
All research outputs
#7,782,070
of 25,382,440 outputs
Outputs from Frontiers in immunology
#9,048
of 31,537 outputs
Outputs of similar age
#128,298
of 350,479 outputs
Outputs of similar age from Frontiers in immunology
#282
of 692 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 31,537 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.4. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 350,479 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.
We're also able to compare this research output to 692 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.