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Validation and Search of the Ideal Cut-Off of the Sysmex UF-1000i® Flow Cytometer for the Diagnosis of Urinary Tract Infection in a Tertiary Hospital in Spain

Overview of attention for article published in Frontiers in Medicine, April 2018
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Title
Validation and Search of the Ideal Cut-Off of the Sysmex UF-1000i® Flow Cytometer for the Diagnosis of Urinary Tract Infection in a Tertiary Hospital in Spain
Published in
Frontiers in Medicine, April 2018
DOI 10.3389/fmed.2018.00092
Pubmed ID
Authors

María I. Millán-Lou, Juan M. García-Lechuz, María A. Ruiz-Andrés, Concepción López, María J. Aldea, María J. Revillo, Antonio Rezusta

Abstract

Urinary tract infections (UTI) are one of the most prevalent infections. A rapid and reliable screening method is useful to screen out negative samples. The objective of this study was to validate the Sysmex flow cytometer UF-1000i by evaluating its accuracy, linearity and carry-over; and define an optimal cut-off value to be used in routine practice in our hospital. For the validation of the UF-1000i cytometer, precision, linearity and carry-over were studied in samples with different counts of bacteria, leukocytes and erythrocytes. Between March and June 2016, urine samples were tested in the Clinical Microbiology Laboratory at University Miguel Servet Hospital, in Spain. Samples were analyzed with the Sysmex UF-1000i cytometer, and cultured. Growth of ≥105 CFUs/mL was considered positive. The validation study reveals that the precision in all the variables is acceptable; that there is a good linearity in the dilutions performed, obtaining values almost identical to those theoretically expected; and for the carry-over has practically null values. A total of 1,220 urine specimens were included, of which 213 (17.4%) were culture positive. The optimal cut-off point of the bacteria-leukocyte combination was 138.8 bacteria or 119.8 leukocytes with an S and E of 95.3 and 70.4%, respectively. The UF-1000i cytometer is a valuable method to screen urine samples to effectively rule out UTI and, may contribute to the reduction of unnecessary urine cultures.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Other 3 20%
Student > Doctoral Student 2 13%
Lecturer > Senior Lecturer 1 7%
Lecturer 1 7%
Professor 1 7%
Other 3 20%
Unknown 4 27%
Readers by discipline Count As %
Medicine and Dentistry 5 33%
Nursing and Health Professions 2 13%
Veterinary Science and Veterinary Medicine 2 13%
Agricultural and Biological Sciences 2 13%
Immunology and Microbiology 1 7%
Other 0 0%
Unknown 3 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 April 2018.
All research outputs
#18,601,965
of 23,041,514 outputs
Outputs from Frontiers in Medicine
#4,025
of 5,806 outputs
Outputs of similar age
#255,568
of 329,292 outputs
Outputs of similar age from Frontiers in Medicine
#95
of 112 outputs
Altmetric has tracked 23,041,514 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,806 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 13.3. This one is in the 13th percentile – i.e., 13% of its peers scored the same or lower than it.
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We're also able to compare this research output to 112 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.