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Tyrosine decarboxylase activity of enterococci grown in media with different nutritional potential: tyramine and 2-phenylethylamine accumulation and tyrDC gene expression

Overview of attention for article published in Frontiers in Microbiology, April 2015
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Title
Tyrosine decarboxylase activity of enterococci grown in media with different nutritional potential: tyramine and 2-phenylethylamine accumulation and tyrDC gene expression
Published in
Frontiers in Microbiology, April 2015
DOI 10.3389/fmicb.2015.00259
Pubmed ID
Authors

Eleonora Bargossi, Giulia Tabanelli, Chiara Montanari, Rosalba Lanciotti, Veronica Gatto, Fausto Gardini, Sandra Torriani

Abstract

The ability to accumulate tyramine and 2-phenylethylamine by two strains of Enterococcus faecalis and two strains Enterococcus faecium was evaluated in two cultural media added or not with tyrosine. All the enterococcal strains possessed a tyrosine decarboxylase (tyrDC) which determined tyramine accumulation in all the conditions tested, independently on the addition of high concentration of free tyrosine. Enterococci differed in rate and level of biogenic amines accumulation. E. faecalis EF37 and E. faecium FC12 produced tyramine in high amount since the exponential growth phase, while 2-phenylethylamine was accumulated when tyrosine was depleted. E. faecium FC12 and E. faecalis ATCC 29212 showed a slower tyraminogenic activity which took place mainly in the stationary phase up to 72 h of incubation. Moreover, E. faecalis ATCC 29212 produced 2-phenylethylamine only in the media without tyrosine added. In BHI added or not with tyrosine the tyrDC gene expression level differed considerably depending on the strains and the growth phase. In particular, the tyrDC gene expression was high during the exponential phase in rich medium for all the strains and subsequently decreased except for E. faecium FC12. Even if tyrDC presence is common among enterococci, this study underlines the extremely variable decarboxylating potential of strains belonging to the same species, suggesting strain-dependent implications in food safety.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 31 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 19%
Other 5 16%
Student > Master 5 16%
Student > Ph. D. Student 3 10%
Student > Bachelor 2 6%
Other 4 13%
Unknown 6 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 39%
Immunology and Microbiology 3 10%
Biochemistry, Genetics and Molecular Biology 3 10%
Medicine and Dentistry 2 6%
Environmental Science 2 6%
Other 3 10%
Unknown 6 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 April 2015.
All research outputs
#18,405,972
of 22,799,071 outputs
Outputs from Frontiers in Microbiology
#19,269
of 24,748 outputs
Outputs of similar age
#192,918
of 264,200 outputs
Outputs of similar age from Frontiers in Microbiology
#276
of 345 outputs
Altmetric has tracked 22,799,071 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,748 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
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