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Genetic and phenotypic evidence of the Salmonella enterica serotype Enteritidis human-animal interface in Chile

Overview of attention for article published in Frontiers in Microbiology, May 2015
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Title
Genetic and phenotypic evidence of the Salmonella enterica serotype Enteritidis human-animal interface in Chile
Published in
Frontiers in Microbiology, May 2015
DOI 10.3389/fmicb.2015.00464
Pubmed ID
Authors

Patricio Retamal, Marcela Fresno, Catherine Dougnac, Sindy Gutierrez, Vanessa Gornall, Roberto Vidal, Rolando Vernal, Myriam Pujol, Marlen Barreto, Daniel González-Acuña, Pedro Abalos

Abstract

Salmonella enterica serotype Enteritidis is a worldwide zoonotic agent that has been recognized as a very important food-borne bacterial pathogen, mainly associated with consumption of poultry products. The aim of this work was to determine genotypic and phenotypic evidence of S. Enteritidis transmission among seabirds, poultry and humans in Chile. Genotyping was performed using PCR-based virulotyping, pulse-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Pathogenicity-associated phenotypes were determined with survival to free radicals, acidic pH, starvation, antimicrobial resistance, and survival within human dendritic cells. As result of PCR and PFGE assays, some isolates from the three hosts showed identical genotypic patterns, and through MLST it was determined that all of them belong to sequence type 11. Phenotypic assays show diversity of bacterial responses among isolates. When results were analyzed according to bacterial host, statistical differences were identified in starvation and dendritic cells survival assays. In addition, isolates from seabirds showed the highest rates of resistance to gentamycin, tetracycline, and ampicillin. Overall, the very close genetic and phenotypic traits shown by isolates from humans, poultry, and seabirds suggest the inter-species transmission of S. Enteritidis bacteria between hosts, likely through anthropogenic environmental contamination that determines infection of seabirds with bacteria that are potentially pathogenic for other susceptible organism, including humans.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 66 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Chile 1 2%
Peru 1 2%
Unknown 64 97%

Demographic breakdown

Readers by professional status Count As %
Student > Master 10 15%
Researcher 8 12%
Student > Ph. D. Student 8 12%
Student > Bachelor 6 9%
Student > Doctoral Student 4 6%
Other 13 20%
Unknown 17 26%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 23%
Medicine and Dentistry 6 9%
Immunology and Microbiology 6 9%
Biochemistry, Genetics and Molecular Biology 6 9%
Veterinary Science and Veterinary Medicine 5 8%
Other 8 12%
Unknown 20 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 June 2015.
All research outputs
#14,810,408
of 22,803,211 outputs
Outputs from Frontiers in Microbiology
#13,783
of 24,751 outputs
Outputs of similar age
#147,455
of 264,753 outputs
Outputs of similar age from Frontiers in Microbiology
#193
of 382 outputs
Altmetric has tracked 22,803,211 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,751 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,753 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 382 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.