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Marinobacter subterrani, a genetically tractable neutrophilic Fe(II)-oxidizing strain isolated from the Soudan Iron Mine

Overview of attention for article published in Frontiers in Microbiology, July 2015
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  • Good Attention Score compared to outputs of the same age (71st percentile)
  • Good Attention Score compared to outputs of the same age and source (75th percentile)

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Title
Marinobacter subterrani, a genetically tractable neutrophilic Fe(II)-oxidizing strain isolated from the Soudan Iron Mine
Published in
Frontiers in Microbiology, July 2015
DOI 10.3389/fmicb.2015.00719
Pubmed ID
Authors

Benjamin M. Bonis, Jeffrey A. Gralnick

Abstract

We report the isolation, characterization, and development of a robust genetic system for a halophilic, Fe(II)-oxidizing bacterium isolated from a vertical borehole originating 714 m below the surface located in the Soudan Iron Mine in northern Minnesota, USA. Sequence analysis of the 16S rRNA gene places the isolate in the genus Marinobacter of the Gammaproteobacteria. The genome of the isolate was sequenced using a combination of short- and long-read technologies resulting in two contigs representing a 4.4 Mbp genome. Using genomic information, we used a suicide vector for targeted deletion of specific flagellin genes, resulting in a motility-deficient mutant. The motility mutant was successfully complemented by expression of the deleted genes in trans. Random mutagenesis using a transposon was also achieved. Capable of heterotrophic growth, this isolate represents a microaerophilic Fe(II)-oxidizing species for which a system for both directed and random mutagenesis has been established. Analysis of 16S rDNA suggests Marinobacter represents a major taxon in the mine, and genetic interrogation of this genus may offer insight into the structure of deep subsurface communities as well as an additional tool for analyzing nutrient and element cycling in the subsurface ecosystem.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 2%
United States 1 2%
Canada 1 2%
Unknown 60 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 30%
Student > Bachelor 8 13%
Researcher 8 13%
Other 6 10%
Student > Master 5 8%
Other 7 11%
Unknown 10 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 19 30%
Biochemistry, Genetics and Molecular Biology 11 17%
Environmental Science 8 13%
Immunology and Microbiology 5 8%
Earth and Planetary Sciences 3 5%
Other 6 10%
Unknown 11 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 November 2015.
All research outputs
#7,515,306
of 26,243,859 outputs
Outputs from Frontiers in Microbiology
#7,162
of 30,145 outputs
Outputs of similar age
#78,953
of 276,933 outputs
Outputs of similar age from Frontiers in Microbiology
#82
of 345 outputs
Altmetric has tracked 26,243,859 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 30,145 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 276,933 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 345 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.