Wajiha Iram, Tehmina Anjum, Mazhar Iqbal, Abdul Ghaffar, Mateen Abbas
Abstract
In this study aqueous extract of seeds and leaves of Trachyspermum ammi were evaluated for their ability to detoxify aflatoxin B1 and B2 (AFB1; 100 μg L(-1) and AFB2; 50 μg L(-1)) by in vitro and in vivo assays. Results indicated that T. ammi seeds extract was found to be significant (P < 0.05) in degrading AFB1 and AFB2 i.e., 92.8 and 91.9% respectively. However, T. ammi leaves extract proved to be less efficient in degrading these aflatoxins, under optimized conditions i.e., pH 8, temperature 30°C and incubation period of 72 h. The structural elucidation of degraded toxin products by LCMS/MS analysis showed that eight degraded products of AFB1 and AFB2 were formed. MS/MS spectra showed that most of the products were formed by the removal of double bond in the terminal furan ring and modification of lactone group indicating less toxicity as compared to parent compounds. Brine shrimps bioassay further confirmed the low toxicity of degraded products, showing that T. ammi seeds extract can be used as an effective tool for the detoxification of aflatoxins.
Pharmacology, Toxicology and Pharmaceutical Science
10
11%
Chemistry
5
6%
Veterinary Science and Veterinary Medicine
4
5%
Medicine and Dentistry
3
3%
Other
14
16%
Unknown
29
33%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 March 2016.
All research outputs
#20,317,110
of 22,858,915 outputs
Outputs from Frontiers in Microbiology
#22,466
of 24,871 outputs
Outputs of similar age
#254,730
of 300,631 outputs
Outputs of similar age from Frontiers in Microbiology
#469
of 545 outputs
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