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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Rapid Detection of Candida albicans by Polymerase Spiral Reaction Assay in Clinical Blood Samples
|
|---|---|
| Published in |
Frontiers in Microbiology, June 2016
|
| DOI | 10.3389/fmicb.2016.00916 |
| Pubmed ID | |
| Authors |
Xiaoqun Jiang, Derong Dong, Lihong Bian, Dayang Zou, Xiaoming He, Da Ao, Zhan Yang, Simo Huang, Ningwei Liu, Wei Liu, Liuyu Huang |
| Abstract |
Candida albicans is the most common human yeast pathogen which causes mucosal infections and invasive fungal diseases. Early detection of this pathogen is needed to guide preventative and therapeutic treatment. The aim of this study was to establish a polymerase spiral reaction (PSR) assay that rapidly and accurately detects C. albicans and to assess the clinical applicability of PSR-based diagnostic testing. Internal transcribed spacer 2 (ITS2), a region between 5.8S and 28S fungal ribosomal DNA, was used as the target sequence. Four primers were designed for amplification of ITS2 with the PSR method, which was evaluated using real time turbidity monitoring and visual detection using a pH indicator. Fourteen non-C. albicans yeast strains were negative for detection, which indicated the specificity of PSR assay was 100%. A 10-fold serial dilution of C. albicans genomic DNA was subjected to PSR and conventional polimerase chain reaction (PCR) to compare their sensitivities. The detection limit of PSR was 6.9 pg/μl within 1 h, 10-fold higher than that of PCR (69.0 pg/μl). Blood samples (n = 122) were collected from intensive care unit and hematological patients with proven or suspected C. albicans infection at two hospitals in Beijing, China. Both PSR assay and the culture method were used to analyze the samples. Of the 122 clinical samples, 34 were identified as positive by PSR. The result was consistent with those obtained by the culture method. In conclusion, a novel and effective C. albicans detection assay was developed that has a great potential for clinical screening and point-of-care testing. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 73 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 1% |
| Unknown | 72 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 11 | 15% |
| Student > Ph. D. Student | 10 | 14% |
| Student > Bachelor | 8 | 11% |
| Researcher | 6 | 8% |
| Other | 4 | 5% |
| Other | 13 | 18% |
| Unknown | 21 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 21% |
| Biochemistry, Genetics and Molecular Biology | 9 | 12% |
| Veterinary Science and Veterinary Medicine | 6 | 8% |
| Medicine and Dentistry | 5 | 7% |
| Immunology and Microbiology | 4 | 5% |
| Other | 9 | 12% |
| Unknown | 25 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 July 2016.
All research outputs
#20,335,423
of 22,880,230 outputs
Outputs from Frontiers in Microbiology
#22,500
of 24,908 outputs
Outputs of similar age
#305,053
of 352,718 outputs
Outputs of similar age from Frontiers in Microbiology
#448
of 525 outputs
Altmetric has tracked 22,880,230 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,908 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,718 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 525 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.