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Identification of Sporopollenin as the Outer Layer of Cell Wall in Microalga Chlorella protothecoides

Overview of attention for article published in Frontiers in Microbiology, June 2016
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105 Mendeley
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Title
Identification of Sporopollenin as the Outer Layer of Cell Wall in Microalga Chlorella protothecoides
Published in
Frontiers in Microbiology, June 2016
DOI 10.3389/fmicb.2016.01047
Pubmed ID
Authors

Xi He, Junbiao Dai, Qingyu Wu

Abstract

Chlorella protothecoides has been put forth as a promising candidate for commercial biodiesel production. However, the cost of biodiesel remains much higher than diesel from fossil fuel sources, partially due to the high costs of oil extraction from algae. Here, we identified the presence of a sporopollenin layer outside the polysaccharide cell wall; this was evaluated using transmission electron microscopy, 2-aminoethanol treatment, acetolysis, and Fourier Transform Infrared Spectroscopy. We also performed bioinformatics analysis of the genes of the C. protothecoides genome that are likely involved in sporopollenin synthesis, secretion, and translocation, and evaluated the expression of these genes via real-time PCR. We also found that that removal of this sporopollenin layer greatly improved the efficiency of oil extraction.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 105 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 105 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 15 14%
Student > Ph. D. Student 14 13%
Student > Master 13 12%
Student > Bachelor 10 10%
Student > Doctoral Student 5 5%
Other 16 15%
Unknown 32 30%
Readers by discipline Count As %
Agricultural and Biological Sciences 28 27%
Biochemistry, Genetics and Molecular Biology 19 18%
Immunology and Microbiology 5 5%
Pharmacology, Toxicology and Pharmaceutical Science 4 4%
Chemical Engineering 4 4%
Other 7 7%
Unknown 38 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 July 2016.
All research outputs
#15,328,720
of 22,880,230 outputs
Outputs from Frontiers in Microbiology
#15,156
of 24,902 outputs
Outputs of similar age
#221,932
of 351,542 outputs
Outputs of similar age from Frontiers in Microbiology
#322
of 514 outputs
Altmetric has tracked 22,880,230 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,902 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 351,542 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 514 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.