Christopher L. Gardner, Fernando A. Pagliai, Lei Pan, Lora Bojilova, Maria I. Torino, Graciela L. Lorca, Claudio F. Gonzalez
Abstract
CLIBASIA_01510, PrbP, is a predicted RNA polymerase binding protein in Liberibacter asiaticus. PrbP was found to regulate expression of a small subset of ribosomal genes through interactions with the β-subunit of the RNA polymerase and a short, specific sequence on the promoter region. Molecular screening assays were performed to identify small molecules that interact with PrbP in vitro. Chemical hits were analyzed for therapeutic efficacy against L. asiaticus via an infected leaf assay, where the transcriptional activity of L. asiaticus was found to decrease significantly after exposure to tolfenamic acid. Similarly, tolfenamic acid was found to inhibit L. asiaticus infection in highly symptomatic citrus seedlings. Our results indicate that PrbP is an important transcriptional regulator for survival of L. asiaticus in planta, and the chemicals identified by molecular screening assays could be used as a therapeutic treatment for huanglongbing disease.
Pharmacology, Toxicology and Pharmaceutical Science
1
2%
Immunology and Microbiology
1
2%
Other
4
9%
Unknown
5
11%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 October 2016.
All research outputs
#18,475,157
of 22,893,031 outputs
Outputs from Frontiers in Microbiology
#19,402
of 24,942 outputs
Outputs of similar age
#239,294
of 316,298 outputs
Outputs of similar age from Frontiers in Microbiology
#300
of 423 outputs
Altmetric has tracked 22,893,031 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,942 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
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We're also able to compare this research output to 423 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.
