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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Virulence Markers and Phylogenetic Analysis of Escherichia coli Strains with Hybrid EAEC/UPEC Genotypes Recovered from Sporadic Cases of Extraintestinal Infections
|
|---|---|
| Published in |
Frontiers in Microbiology, February 2017
|
| DOI | 10.3389/fmicb.2017.00146 |
| Pubmed ID | |
| Authors |
Flaviane B. M. Lara, Danielly R. Nery, Pâmela M. de Oliveira, Mayana L. Araujo, Fabiana R. Q. Carvalho, Lorena C. F. Messias-Silva, Leonardo B. Ferreira, Celio Faria-Junior, Alex L. Pereira |
| Abstract |
Virulence genes from different E. coli pathotypes are blended in hybrid strains. E. coli strains with hybrid enteroaggregative/uropathogenic (EAEC/UPEC) genotypes have sporadically emerged causing outbreaks of extraintestinal infections, however their association with routine infections is yet underappreciated. We assessed 258 isolates of E. coli recovered from 86 consecutive cases of extraintestinal infections seeking EAEC and hybrid genotype (EAEC/UPEC) strains. Extensive virulence genotyping was carried out to detect 21 virulence genes, including molecular predictors of EAEC and UPEC strains. Phylogenetic groups and sequence types (STs) were identified, as well as it was performed phylogenetic analyses in order to evaluate whether hybrid EAEC/UPEC strains belonged to intestinal or extraintestinal lineages of E. coli. Adhesion assays were performed to evaluate the biofilm formation by hybrid strains in human urine and cell culture medium (DMEM). Molecular predictors of UPEC were detected in more than 70% of the strains (chuA in 85% and fyuA in 78%). Otherwise, molecular predictors of EAEC (aatA and aggR) were detected in only 3.4% (9/258) of the strains and always along with the UPEC predictor fyuA. Additionally, the pyelonephritis-associated pilus (pap) gene was also detected in all of the hybrid EAEC/UPEC strains. EAEC/UPEC strains were recovered from two cases of community-onset urinary tract infections (UTI) and from a case of bacteremia. Analyses revealed that hybrid EAEC/UPEC strains were phylogenetically positioned in two different clades. Two representative strains, each recovered from UTI and bacteremia, were positioned into a characteristic UPEC clade marked by strains belonging to phylogenetic group D and ST3 (Warwick ST 69). Another hybrid EAEC/UPEC strain was classified as phylogroup A-ST478 and positioned in a commensal clade. Hybrid EAEC/UPEC strains formed biofilms at modest, but perceptible levels either in DMEM or in urine samples. We showed that different lineages of E. coli, at least phylogenetic group A and D, can acquire and gather EAEC and UPEC virulence genes promoting the emergence of hybrid EAEC/UPEC strains. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 92 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 92 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 17 | 18% |
| Student > Ph. D. Student | 13 | 14% |
| Student > Master | 13 | 14% |
| Researcher | 9 | 10% |
| Student > Postgraduate | 7 | 8% |
| Other | 12 | 13% |
| Unknown | 21 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 27 | 29% |
| Biochemistry, Genetics and Molecular Biology | 16 | 17% |
| Agricultural and Biological Sciences | 7 | 8% |
| Medicine and Dentistry | 6 | 7% |
| Engineering | 5 | 5% |
| Other | 6 | 7% |
| Unknown | 25 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2017.
All research outputs
#15,431,277
of 22,940,083 outputs
Outputs from Frontiers in Microbiology
#15,246
of 24,976 outputs
Outputs of similar age
#256,984
of 420,674 outputs
Outputs of similar age from Frontiers in Microbiology
#302
of 425 outputs
Altmetric has tracked 22,940,083 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,976 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
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