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Identification of an Alternative rRNA Post-transcriptional Maturation of 26S rRNA in the Kingdom Fungi

Overview of attention for article published in Frontiers in Microbiology, May 2018
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Title
Identification of an Alternative rRNA Post-transcriptional Maturation of 26S rRNA in the Kingdom Fungi
Published in
Frontiers in Microbiology, May 2018
DOI 10.3389/fmicb.2018.00994
Pubmed ID
Authors

Alfonso Navarro-Ródenas, Andrea Carra, Asunción Morte

Abstract

Despite of the integrity of their RNA, some desert truffles present a non-canonical profile of rRNA where 3.3 kb is absent, 1.8 kb is clear and a band of 1.6 kb is observed. A similar rRNA profile was identified in organisms belonging to different life kingdoms, with the exception of the Kingdom Fungi, as a result of a split LSU rRNA called hidden gap. rRNA profiles of desert truffles were analyzed to verify the presence of the non-canonical profile. The RNA of desert truffles and yeast were blotted and hybridized with probes complementary to LSU extremes. RACE of LSU rRNA was carried out to determine the LSU rRNA breakage point. LSU rRNA of desert truffles presents a post-transcriptional cleavage of five nucleotides that generates a hidden gap located in domain D7. LSU splits into two molecules of 1.6 and 1.8 kb. Similar to other organisms, a UAAU tract, downstream of the breakage point, was identified. Phylogenetic comparison suggests that during fungi evolution mutations were introduced in the hypervariable D7 domain, resulting in a sequence that is specifically post-transcriptionally cleaved in some desert truffles.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 28%
Student > Ph. D. Student 2 11%
Other 1 6%
Student > Doctoral Student 1 6%
Professor 1 6%
Other 3 17%
Unknown 5 28%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 39%
Biochemistry, Genetics and Molecular Biology 5 28%
Unknown 6 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 June 2018.
All research outputs
#20,522,137
of 23,090,520 outputs
Outputs from Frontiers in Microbiology
#22,842
of 25,257 outputs
Outputs of similar age
#290,352
of 330,791 outputs
Outputs of similar age from Frontiers in Microbiology
#557
of 644 outputs
Altmetric has tracked 23,090,520 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 25,257 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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