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Study the Features of 57 Confirmed CRISPR Loci in 38 Strains of Staphylococcus aureus

Overview of attention for article published in Frontiers in Microbiology, July 2018
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Title
Study the Features of 57 Confirmed CRISPR Loci in 38 Strains of Staphylococcus aureus
Published in
Frontiers in Microbiology, July 2018
DOI 10.3389/fmicb.2018.01591
Pubmed ID
Authors

Xihong Zhao, Zhixue Yu, Zhenbo Xu

Abstract

Staphylococcus aureus is a foodborne pathogen that causes food contamination and food poisoning, which poses great harm to health, agriculture and other hosts. Clustered regularly interspaced short palindromic repeats (CRISPR) are a recently discovered bacterial immune system that resists foreign genes such as phage DNA. This system inhibits the transfer of specific movable genetic elements that match the CRISPR spacer sequences, thereby preventing the spread of drug-resistant genes between pathogens. In this study, 57 CRISPR loci were screened from 38 strains of S. aureus based on the CRISPR database, and bioinformatics tools were used to investigate the structural features and potential functions of S. aureus CRISPR loci. The results showed that most strains contained only one CRISPR locus, a few strains contained multiple loci with sparsely distributed sites. These loci mainly included highly conserved direct repeat sequences and highly variable spacer sequences, as well as polymorphic cas genes. In addition, the analysis of secondary structure of direct repeat RNA showed that all sites can form stable RNA secondary structure. The results of constructing phylogenetic tree based on spacer sequence showed that some strains contained a high degree of phylogenetic relationship, while the differences among other strains in evolutionary processes were quite obvious. Of the 57 CRISPR loci identified, only the cas gene was found near the 4 CRISPR loci.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 53 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 21%
Student > Ph. D. Student 7 13%
Student > Bachelor 6 11%
Student > Master 4 8%
Student > Postgraduate 3 6%
Other 5 9%
Unknown 17 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 19%
Agricultural and Biological Sciences 7 13%
Immunology and Microbiology 6 11%
Engineering 2 4%
Environmental Science 1 2%
Other 7 13%
Unknown 20 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 August 2018.
All research outputs
#15,014,589
of 23,096,849 outputs
Outputs from Frontiers in Microbiology
#14,033
of 25,270 outputs
Outputs of similar age
#198,619
of 330,319 outputs
Outputs of similar age from Frontiers in Microbiology
#434
of 741 outputs
Altmetric has tracked 23,096,849 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 25,270 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 330,319 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 741 others from the same source and published within six weeks on either side of this one. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.