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Excision and Circularization of Integrative Conjugative Element Tn5253 of Streptococcus pneumoniae

Overview of attention for article published in Frontiers in Microbiology, July 2018
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Title
Excision and Circularization of Integrative Conjugative Element Tn5253 of Streptococcus pneumoniae
Published in
Frontiers in Microbiology, July 2018
DOI 10.3389/fmicb.2018.01779
Pubmed ID
Authors

Francesco Santoro, Alessandra Romeo, Gianni Pozzi, Francesco Iannelli

Abstract

The integrative conjugative element (ICE) Tn5253 of Streptococcus pneumoniae, conferring resistance to tetracycline and chloramphenicol, was found integrated at a 83-bp specific target site (attB) located in the rbgA gene of the pneumococcal chromosome. PCR analysis of Tn5253-carrying strains showed evidence of precise excision of Tn5253 from the pneumococcal chromosome with production of (i) circular forms of the ICE in which the ends were joined by a 84-bp sequence (attTn), and (ii) reconstituted chromosomal attB. When integrated into the chromosome, Tn5253 was flanked by attL, identical to attB, and attR, identical to attTn. Circular forms of Tn5253 were present at a concentration of 3.8 × 10-4 copies per chromosome, whereas reconstituted attB sites were at 3.0 × 10-4 copies per chromosome. Deletion of int-xis of Tn5253 abolished production of circular forms (<7.1 × 10-6 copies per chromosome) and was associated to the lack of Tn5253 conjugal transfer suggesting, as expected, that Tn5253 circular form acts as a conjugation intermediate.

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Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 29%
Student > Ph. D. Student 4 19%
Student > Master 3 14%
Professor > Associate Professor 1 5%
Unknown 7 33%
Readers by discipline Count As %
Immunology and Microbiology 5 24%
Biochemistry, Genetics and Molecular Biology 3 14%
Agricultural and Biological Sciences 2 10%
Veterinary Science and Veterinary Medicine 1 5%
Unknown 10 48%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2018.
All research outputs
#18,647,094
of 23,100,534 outputs
Outputs from Frontiers in Microbiology
#19,675
of 25,279 outputs
Outputs of similar age
#253,590
of 329,832 outputs
Outputs of similar age from Frontiers in Microbiology
#561
of 743 outputs
Altmetric has tracked 23,100,534 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 25,279 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
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We're also able to compare this research output to 743 others from the same source and published within six weeks on either side of this one. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.