Title |
Further Elucidation of Galactose Utilization in Lactococcus lactis MG1363
|
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Published in |
Frontiers in Microbiology, August 2018
|
DOI | 10.3389/fmicb.2018.01803 |
Pubmed ID | |
Authors |
Ana Solopova, Herwig Bachmann, Bas Teusink, Jan Kok, Oscar P. Kuipers |
Abstract |
Since the 1970s, galactose metabolism in Lactococcus lactis has been in debate. Different studies led to diverse outcomes making it difficult to conclude whether galactose uptake was PEP- or ATP- dependent and decide what the exact connection was between galactose and lactose uptake and metabolism. It was shown that some Lactococcus strains possess two galactose-specific systems - a permease and a PTS, even if they lack the lactose utilization plasmid, proving that a lactose-independent PTSGal exists. However, the PTSGal transporter was never identified. Here, with the help of transcriptome analyses and genetic knock-out mutants, we reveal the identities of two low-affinity galactose PTSs. A novel plant-niche-related PTS component Llmg_0963 forming a hybrid transporter Llmg_0963PtcBA and a glucose/mannose-specific PTS are shown to be involved in galactose transport in L. lactis MG1363. |
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Geographical breakdown
Country | Count | As % |
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India | 1 | 20% |
Netherlands | 1 | 20% |
Unknown | 3 | 60% |
Demographic breakdown
Type | Count | As % |
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Scientists | 3 | 60% |
Members of the public | 2 | 40% |
Mendeley readers
Geographical breakdown
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Unknown | 54 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 13 | 24% |
Researcher | 9 | 17% |
Student > Bachelor | 8 | 15% |
Student > Master | 4 | 7% |
Student > Postgraduate | 3 | 6% |
Other | 0 | 0% |
Unknown | 17 | 31% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 17 | 31% |
Agricultural and Biological Sciences | 8 | 15% |
Immunology and Microbiology | 4 | 7% |
Engineering | 2 | 4% |
Chemistry | 2 | 4% |
Other | 4 | 7% |
Unknown | 17 | 31% |