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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Cryo-EM Grid Preparation of Membrane Protein Samples for Single Particle Analysis
|
|---|---|
| Published in |
Frontiers in Molecular Biosciences, July 2018
|
| DOI | 10.3389/fmolb.2018.00074 |
| Pubmed ID | |
| Authors |
Germán G. Sgro, Tiago R. D. Costa |
| Abstract |
Recent advances in cryo-electron microscopy (cryo-EM) have made it possible to solve structures of biological macromolecules at near atomic resolution. Development of more stable microscopes, improved direct electron detectors and faster software for image processing has enabled structural solution of not only large macromolecular (megadalton range) complexes but also small (~60 kDa) proteins. As a result of the widespread use of the technique, we have also witnessed new developments of techniques for cryo-EM grid preparation of membrane protein samples. This includes new types of solubilization strategies that better stabilize these protein complexes and the development of new grid supports with proven efficacy in reducing the motion of the molecules during electron beam exposure. Here, we discuss the practicalities and recent challenges of membrane protein sample preparation and vitrification, as well as grid support and foil treatment in the context of the structure determination of protein complexes by single particle cryo-EM. |
X Demographics
The data shown below were collected from the profiles of 9 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Belgium | 1 | 11% |
| Australia | 1 | 11% |
| Singapore | 1 | 11% |
| United Kingdom | 1 | 11% |
| Unknown | 5 | 56% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 5 | 56% |
| Members of the public | 4 | 44% |
Mendeley readers
The data shown below were compiled from readership statistics for 331 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 331 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 77 | 23% |
| Researcher | 57 | 17% |
| Student > Bachelor | 39 | 12% |
| Student > Master | 35 | 11% |
| Student > Doctoral Student | 20 | 6% |
| Other | 25 | 8% |
| Unknown | 78 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 138 | 42% |
| Agricultural and Biological Sciences | 39 | 12% |
| Chemistry | 24 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 7 | 2% |
| Engineering | 6 | 2% |
| Other | 31 | 9% |
| Unknown | 86 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 April 2022.
All research outputs
#2,697,004
of 23,523,017 outputs
Outputs from Frontiers in Molecular Biosciences
#176
of 4,091 outputs
Outputs of similar age
#55,319
of 330,782 outputs
Outputs of similar age from Frontiers in Molecular Biosciences
#4
of 27 outputs
Altmetric has tracked 23,523,017 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,091 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 330,782 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.