Title |
Augmentation of Ca2+ signaling in astrocytic endfeet in the latent phase of temporal lobe epilepsy
|
---|---|
Published in |
Frontiers in Cellular Neuroscience, February 2015
|
DOI | 10.3389/fncel.2015.00049 |
Pubmed ID | |
Authors |
Karolina Szokol, Kjell Heuser, Wannan Tang, Vidar Jensen, Rune Enger, Peter Bedner, Christian Steinhäuser, Erik Taubøll, Ole Petter Ottersen, Erlend A. Nagelhus |
Abstract |
Astrocytic endfeet are specialized cell compartments whose important homeostatic roles depend on their enrichment of water and ion channels anchored by the dystrophin associated protein complex (DAPC). This protein complex is known to disassemble in patients with mesial temporal lobe epilepsy and in the latent phase of experimental epilepsies. The mechanistic underpinning of this disassembly is an obvious target of future therapies, but remains unresolved. Here we show in a kainate model of temporal lobe epilepsy that astrocytic endfeet display an enhanced stimulation-evoked Ca(2+) signal that outlast the Ca(2+) signal in the cell bodies. While the amplitude of this Ca(2+) signal is reduced following group I/II metabotropic receptor (mGluR) blockade, the duration is sustained. Based on previous studies it has been hypothesized that the molecular disassembly in astrocytic endfeet is caused by dystrophin cleavage mediated by Ca(2+) dependent proteases. Using a newly developed genetically encoded Ca(2+) sensor, the present study bolsters this hypothesis by demonstrating long-lasting, enhanced stimulation-evoked Ca(2+) signals in astrocytic endfeet. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Switzerland | 1 | 2% |
Unknown | 51 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 11 | 21% |
Researcher | 11 | 21% |
Student > Bachelor | 5 | 10% |
Professor | 4 | 8% |
Professor > Associate Professor | 4 | 8% |
Other | 8 | 15% |
Unknown | 9 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 18 | 35% |
Agricultural and Biological Sciences | 14 | 27% |
Medicine and Dentistry | 7 | 13% |
Physics and Astronomy | 1 | 2% |
Biochemistry, Genetics and Molecular Biology | 1 | 2% |
Other | 1 | 2% |
Unknown | 10 | 19% |