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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Structural plasticity of spines at giant mossy fiber synapses
|
|---|---|
| Published in |
Frontiers in Neural Circuits, January 2012
|
| DOI | 10.3389/fncir.2012.00103 |
| Pubmed ID | |
| Authors |
Shanting Zhao, Daniel Studer, Xuejun Chai, Werner Graber, Nils Brose, Sigrun Nestel, Christina Young, E. Patricia Rodriguez, Kurt Saetzler, Michael Frotscher |
| Abstract |
The granule cells of the dentate gyrus give rise to thin unmyelinated axons, the mossy fibers. They form giant presynaptic boutons impinging on large complex spines on the proximal dendritic portions of hilar mossy cells and CA3 pyramidal neurons. While these anatomical characteristics have been known for some time, it remained unclear whether functional changes at mossy fiber synapses such as long-term potentiation (LTP) are associated with structural changes. Since subtle structural changes may escape a fine-structural analysis when the tissue is fixed by using aldehydes and is dehydrated in ethanol, rapid high-pressure freezing (HPF) of the tissue was applied. Slice cultures of hippocampus were prepared and incubated in vitro for 2 weeks. Then, chemical LTP (cLTP) was induced by the application of 25 mM tetraethylammonium (TEA) for 10 min. Whole-cell patch-clamp recordings from CA3 pyramidal neurons revealed a highly significant potentiation of mossy fiber synapses when compared to control conditions before the application of TEA. Next, the slice cultures were subjected to HPF, cryosubstitution, and embedding in Epon for a fine-structural analysis. When compared to control tissue, we noticed a significant decrease of synaptic vesicles in mossy fiber boutons and a concomitant increase in the length of the presynaptic membrane. On the postsynaptic side, we observed the formation of small, finger-like protrusions, emanating from the large complex spines. These short protrusions gave rise to active zones that were shorter than those normally found on the thorny excrescences. However, the total number of active zones was significantly increased. Of note, none of these cLTP-induced structural changes was observed in slice cultures from Munc13-1 deficient mouse mutants showing severely impaired vesicle priming and docking. In conclusion, application of HPF allowed us to monitor cLTP-induced structural reorganization of mossy fiber synapses. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | 3% |
| Indonesia | 1 | 1% |
| Canada | 1 | 1% |
| Unknown | 68 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 20 | 28% |
| Researcher | 18 | 25% |
| Student > Doctoral Student | 9 | 13% |
| Student > Master | 4 | 6% |
| Student > Bachelor | 3 | 4% |
| Other | 8 | 11% |
| Unknown | 10 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 30 | 42% |
| Neuroscience | 16 | 22% |
| Medicine and Dentistry | 7 | 10% |
| Biochemistry, Genetics and Molecular Biology | 4 | 6% |
| Psychology | 2 | 3% |
| Other | 2 | 3% |
| Unknown | 11 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 December 2012.
All research outputs
#20,176,348
of 22,689,790 outputs
Outputs from Frontiers in Neural Circuits
#1,026
of 1,209 outputs
Outputs of similar age
#221,229
of 244,142 outputs
Outputs of similar age from Frontiers in Neural Circuits
#44
of 73 outputs
Altmetric has tracked 22,689,790 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,209 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 73 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.