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Mendeley readers
Attention Score in Context
| Title |
Chemical Stimulation of Adherent Cells by Localized Application of Acetylcholine from a Microfluidic System
|
|---|---|
| Published in |
Frontiers in Neuroengineering, January 2010
|
| DOI | 10.3389/fneng.2010.00113 |
| Pubmed ID | |
| Authors |
Susanne Zibek, Britta Hagmeyer, Alfred Stett, Martin Stelzle |
| Abstract |
Chemical stimulation of cells is inherently cell type selective in contrast to electro-stimulation. The availability of a system for localized application of minute amounts of chemical stimulants could be useful for dose related response studies to test new compounds. It could also bring forward the development of a novel type of neuroprostheses. In an experimental setup microdroplets of an acetylcholine solution were ejected from a fluidic microsystem and applied to the bottom of a nanoporous membrane. The solution traveled through the pores to the top of the membrane on which TE671 cells were cultivated. Calcium imaging was used to visualize cellular response with temporal and spatial resolution. Experimental demonstration of chemical stimulation for both threshold gated stimulation as well as accumulated dose-response was achieved by either employing acetylcholine as chemical stimulant or applying calcein uptake, respectively. Numerical modeling and simulation of transport mechanisms involved were employed to gain a theoretical understanding of the influence of pore size, concentration of stimulant and droplet volume on the spatial-temporal distribution of stimulant and on the cellular response. Diffusion, pressure driven flow and evaporation effects were taken into account. Fast stimulation kinetic is achieved with pores of 0.82 μm diameter, whereas sustained substance delivery is obtained with nanoporous membranes. In all cases threshold concentrations ranging from 0.01 to 0.015 μM acetylcholine independent of pore size were determined. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | 12% |
| Kazakhstan | 1 | 6% |
| China | 1 | 6% |
| Switzerland | 1 | 6% |
| Unknown | 12 | 71% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 35% |
| Researcher | 6 | 35% |
| Student > Master | 2 | 12% |
| Student > Doctoral Student | 1 | 6% |
| Lecturer | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 1 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 8 | 47% |
| Medicine and Dentistry | 2 | 12% |
| Physics and Astronomy | 2 | 12% |
| Unspecified | 1 | 6% |
| Agricultural and Biological Sciences | 1 | 6% |
| Other | 2 | 12% |
| Unknown | 1 | 6% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 April 2011.
All research outputs
#20,165,369
of 22,675,759 outputs
Outputs from Frontiers in Neuroengineering
#70
of 82 outputs
Outputs of similar age
#156,597
of 163,537 outputs
Outputs of similar age from Frontiers in Neuroengineering
#6
of 7 outputs
Altmetric has tracked 22,675,759 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 82 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 163,537 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one.