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Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster

Overview of attention for article published in Frontiers in Molecular Neuroscience, December 2016
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Title
Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster
Published in
Frontiers in Molecular Neuroscience, December 2016
DOI 10.3389/fnmol.2016.00148
Pubmed ID
Authors

Soomin Kim, Kelly Tellez, Graham Buchan, Tim Lebestky

Abstract

Optomotor behavior represents a stereotyped locomotor response to visual motion that is found in both vertebrate and invertebrate models. The Fly Stampede assay was developed to study an optomotor response in freely walking populations of Drosophila. Here we share optimized assay designs and software for production of a modified stampede assay that can be used for genetic screens, and improved tracking outputs for understanding behavioral parameters of visual-motion responses and arousal state of individual animals. Arousal state influences behavioral performance in the stampede assay. As proof of principle experiments we show parametric modulation of visual stimuli and startle stimuli in both wildtype and mutant flies for the type I family dopamine receptor Dop1R1 (DopR). DopR mutants are hyperactive and perform poorly in the stampede assay, suggesting a potential role in visual perception and/or arousal. The stampede assay creates an efficient platform for rapid screening of mutant animals or circuit manipulations for investigating attentional processes in Drosophila.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 32%
Researcher 3 16%
Student > Doctoral Student 2 11%
Other 2 11%
Student > Bachelor 1 5%
Other 3 16%
Unknown 2 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 42%
Neuroscience 6 32%
Biochemistry, Genetics and Molecular Biology 2 11%
Medicine and Dentistry 1 5%
Unknown 2 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 January 2017.
All research outputs
#20,376,559
of 22,925,760 outputs
Outputs from Frontiers in Molecular Neuroscience
#2,483
of 2,895 outputs
Outputs of similar age
#355,573
of 421,042 outputs
Outputs of similar age from Frontiers in Molecular Neuroscience
#71
of 84 outputs
Altmetric has tracked 22,925,760 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,895 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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