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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Using SSR-HRM to Identify Closely Related Species in Herbal Medicine Products: A Case Study on Licorice
|
|---|---|
| Published in |
Frontiers in Pharmacology, April 2018
|
| DOI | 10.3389/fphar.2018.00407 |
| Pubmed ID | |
| Authors |
Jingjian Li, Chao Xiong, Xia He, Zhaocen Lu, Xin Zhang, Xiaoyang Chen, Wei Sun |
| Abstract |
Traditional herbal medicines have played important roles in the ways of life of people around the world since ancient times. Despite the advanced medical technology of the modern world, herbal medicines are still used as popular alternatives to synthetic drugs. Due to the increasing demand for herbal medicines, plant species identification has become an important tool to prevent substitution and adulteration. Here we propose a method for biological assessment of the quality of prescribed species in the Chinese Pharmacopoeia by use of high resolution melting (HRM) analysis of microsatellite loci. We tested this method on licorice, a traditional herbal medicine with a long history. Results showed that nine simple sequence repeat (SSR) markers produced distinct melting curve profiles for the five licorice species investigated using HRM analysis. These results were validated by capillary electrophoresis. We applied this protocol to commercially available licorice products, thus enabling the consistent identification of 11 labels with non-declared Glycyrrhiza species. This novel strategy may thus facilitate DNA barcoding as a method of identification of closely related species in herbal medicine products. Based on this study, a brief operating procedure for using the SSR-HRM protocol for herbal authentication is provided. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Ecuador | 1 | 50% |
| Switzerland | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 35 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 29% |
| Student > Master | 4 | 11% |
| Student > Ph. D. Student | 2 | 6% |
| Student > Doctoral Student | 2 | 6% |
| Student > Bachelor | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 13 | 37% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 37% |
| Biochemistry, Genetics and Molecular Biology | 4 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
| Neuroscience | 2 | 6% |
| Social Sciences | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 13 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 April 2019.
All research outputs
#17,947,156
of 23,045,021 outputs
Outputs from Frontiers in Pharmacology
#7,224
of 16,374 outputs
Outputs of similar age
#236,907
of 326,487 outputs
Outputs of similar age from Frontiers in Pharmacology
#160
of 395 outputs
Altmetric has tracked 23,045,021 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 16,374 research outputs from this source. They receive a mean Attention Score of 5.0. This one is in the 48th percentile – i.e., 48% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 326,487 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 395 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.