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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Nitrate reductase 15N discrimination in Arabidopsis thaliana, Zea mays, Aspergillus niger, Pichea angusta, and Escherichia coli
|
|---|---|
| Published in |
Frontiers in Plant Science, July 2014
|
| DOI | 10.3389/fpls.2014.00317 |
| Pubmed ID | |
| Authors |
Eli Carlisle, Chris Yarnes, Michael D. Toney, Arnold J. Bloom |
| Abstract |
Stable (15)N isotopes have been used to examine movement of nitrogen (N) through various pools of the global N cycle. A central reaction in the cycle involves the reduction of nitrate (NO(-) 3) to nitrite (NO(-) 2) catalyzed by nitrate reductase (NR). Discrimination against (15)N by NR is a major determinant of isotopic differences among N pools. Here, we measured in vitro (15)N discrimination by several NRs purified from plants, fungi, and a bacterium to determine the intrinsic (15)N discrimination by the enzyme and to evaluate the validity of measurements made using (15)N-enriched NO(-) 3. Observed NR isotope discrimination ranged from 22 to 32‰ (kinetic isotope effects of 1.022-1.032) among the different isozymes at natural abundance (15)N (0.37%). As the fractional (15)N content of substrate NO(-) 3 increased from natural abundance, the product (15)N fraction deviated significantly from that expected based on substrate enrichment and (15)N discrimination measured at natural abundance. Additionally, isotopic discrimination by denitrifying bacteria used to reduce NO(-) 3 and NO(-) 2 in some protocols became a greater source of error as (15)N enrichment increased. We briefly discuss potential causes of the experimental artifacts with enriched (15)N and recommend against the use of highly enriched (15)N tracers to study N discrimination in plants or soils. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 2% |
| Unknown | 42 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 13 | 30% |
| Student > Ph. D. Student | 7 | 16% |
| Student > Bachelor | 3 | 7% |
| Researcher | 3 | 7% |
| Student > Doctoral Student | 2 | 5% |
| Other | 6 | 14% |
| Unknown | 9 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 42% |
| Environmental Science | 4 | 9% |
| Biochemistry, Genetics and Molecular Biology | 3 | 7% |
| Earth and Planetary Sciences | 2 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
| Other | 4 | 9% |
| Unknown | 11 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 July 2014.
All research outputs
#20,232,430
of 22,758,248 outputs
Outputs from Frontiers in Plant Science
#15,959
of 20,059 outputs
Outputs of similar age
#192,117
of 227,685 outputs
Outputs of similar age from Frontiers in Plant Science
#118
of 167 outputs
Altmetric has tracked 22,758,248 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,059 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 227,685 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 167 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.