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Transformation and Evaluation of Cry1Ac+Cry2A and GTGene in Gossypium hirsutum L.

Overview of attention for article published in Frontiers in Plant Science, November 2015
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Title
Transformation and Evaluation of Cry1Ac+Cry2A and GTGene in Gossypium hirsutum L.
Published in
Frontiers in Plant Science, November 2015
DOI 10.3389/fpls.2015.00943
Pubmed ID
Authors

Agung N. Puspito, Abdul Q. Rao, Muhammad N. Hafeez, Muhammad S. Iqbal, Kamran S. Bajwa, Qurban Ali, Bushra Rashid, Muhammad A. Abbas, Ayesha Latif, Ahmad A. Shahid, Idrees A. Nasir, Tayyab Husnain

Abstract

More than 50 countries around the globe cultivate cotton on a large scale. It is a major cash crop of Pakistan and is considered "white gold" because it is highly important to the economy of Pakistan. In addition to its importance, cotton cultivation faces several problems, such as insect pests, weeds, and viruses. In the past, insects have been controlled by insecticides, but this method caused a severe loss to the economy. However, conventional breeding methods have provided considerable breakthroughs in the improvement of cotton, but it also has several limitations. In comparison with conventional methods, biotechnology has the potential to create genetically modified plants that are environmentally safe and economically viable. In this study, a local cotton variety VH 289 was transformed with two Bt genes (Cry1Ac and Cry2A) and a herbicide resistant gene (cp4 EPSPS) using the Agrobacterium mediated transformation method. The constitutive CaMV 35S promoter was attached to the genes taken from Bacillus thuringiensis (Bt) and to an herbicide resistant gene during cloning, and this promoter was used for the expression of the genes in cotton plants. This construct was used to develop the Glyphosate Tolerance Gene (GTGene) for herbicide tolerance and insecticidal gene (Cry1Ac and Cry2A) for insect tolerance in the cotton variety VH 289. The transgenic cotton variety performed 85% better compared with the non-transgenic variety. The study results suggest that farmers should use the transgenic cotton variety for general cultivation to improve the production of cotton.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 3%
Unknown 36 97%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 9 24%
Student > Ph. D. Student 8 22%
Student > Master 3 8%
Researcher 2 5%
Student > Postgraduate 2 5%
Other 5 14%
Unknown 8 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 32%
Biochemistry, Genetics and Molecular Biology 10 27%
Chemistry 2 5%
Environmental Science 1 3%
Social Sciences 1 3%
Other 1 3%
Unknown 10 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 November 2015.
All research outputs
#20,295,501
of 22,832,057 outputs
Outputs from Frontiers in Plant Science
#16,042
of 20,146 outputs
Outputs of similar age
#236,666
of 282,567 outputs
Outputs of similar age from Frontiers in Plant Science
#292
of 379 outputs
Altmetric has tracked 22,832,057 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,146 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 282,567 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 379 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.