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Quantitative Profiling of Feruloylated Arabinoxylan Side-Chains from Graminaceous Cell Walls

Overview of attention for article published in Frontiers in Plant Science, January 2016
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Title
Quantitative Profiling of Feruloylated Arabinoxylan Side-Chains from Graminaceous Cell Walls
Published in
Frontiers in Plant Science, January 2016
DOI 10.3389/fpls.2015.01249
Pubmed ID
Authors

Rachel R. Schendel, Marleen R. Meyer, Mirko Bunzel

Abstract

Graminaceous arabinoxylans are distinguished by decoration with feruloylated monosaccharidic and oligosaccharidic side-chains. Although it is hypothesized that structural complexity and abundance of these feruloylated arabinoxylan side-chains may contribute, among other factors, to resistance of plant cell walls to enzymatic degradation, quantitative profiling approaches for these structural units in plant cell wall materials have not been described yet. Here we report the development and application of a rapid and robust method enabling the quantitative comparison of feruloylated side-chain profiles in cell wall materials following mildly acidic hydrolysis, C18-solid phase extraction (SPE), reduction under aprotic conditions, and liquid chromatography with diode-array detection/mass spectrometry (LC-DAD/MS) separation and detection. The method was applied to the insoluble fiber/cell wall materials isolated from 12 whole grains: wild rice (Zizania aquatica L.), long-grain brown rice (Oryza sativa L.), rye (Secale cereale L.), kamut (Triticum turanicum Jakubz.), wheat (Triticum aestivum L.), spelt (Triticum spelta L.), intermediate wheatgrass (Thinopyrum intermedium), maize (Zea mays L.), popcorn (Zea mays L. var. everta), oat (Avena sativa L.) (dehulled), barley (Hordeum vulgare L.) (dehulled), and proso millet (Panicum miliaceum L.). Between 51 and 96% of the total esterified monomeric ferulates were represented in the quantified compounds captured in the feruloylated side-chain profiles, which confirms the significance of these structures to the global arabinoxylan structure in terms of quantity. The method provided new structural insights into cereal grain arabinoxylans, in particular, that the structural moiety α-l-galactopyranosyl-(1→2)-β-d-xylopyranosyl-(1→2)-5-O-trans-feruloyl-l-arabinofuranose (FAXG), which had previously only been described in maize, is ubiquitous to cereal grains.

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Mendeley readers

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The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 1%
Unknown 66 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 20 30%
Researcher 10 15%
Student > Master 7 10%
Student > Bachelor 4 6%
Other 3 4%
Other 6 9%
Unknown 17 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 27 40%
Biochemistry, Genetics and Molecular Biology 7 10%
Chemistry 4 6%
Chemical Engineering 2 3%
Nursing and Health Professions 1 1%
Other 4 6%
Unknown 22 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2016.
All research outputs
#17,780,575
of 22,840,638 outputs
Outputs from Frontiers in Plant Science
#12,030
of 20,160 outputs
Outputs of similar age
#269,009
of 395,720 outputs
Outputs of similar age from Frontiers in Plant Science
#229
of 466 outputs
Altmetric has tracked 22,840,638 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,160 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 395,720 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 466 others from the same source and published within six weeks on either side of this one. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.