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P1 Epigenetic Regulation in Leaves of High Altitude Maize Landraces: Effect of UV-B Radiation

Overview of attention for article published in Frontiers in Plant Science, April 2016
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Title
P1 Epigenetic Regulation in Leaves of High Altitude Maize Landraces: Effect of UV-B Radiation
Published in
Frontiers in Plant Science, April 2016
DOI 10.3389/fpls.2016.00523
Pubmed ID
Authors

Sebastián P. Rius, Julia Emiliani, Paula Casati

Abstract

P1 is a R2R3-MYB transcription factor that regulates the accumulation of a specific group of flavonoids in maize floral tissues, such as flavones and phlobaphenes. P1 is also highly expressed in leaves of maize landraces adapted to high altitudes and higher levels of UV-B radiation. In this work, we analyzed the epigenetic regulation of the P1 gene by UV-B in leaves of different maize landraces. Our results demonstrate that DNA methylation in the P1 proximal promoter, intron1 and intron2 is decreased by UV-B in all lines analyzed; however, the basal DNA methylation levels are lower in the landraces than in B73, a low altitude inbred line. DNA demethylation by UV-B is accompanied by a decrease in H3 methylation at Lys 9 and 27, and by an increase in H3 acetylation. smRNAs complementary to specific regions of the proximal promoter and of intron 2 3' end are also decreased by UV-B; interestingly, P1 smRNA levels are lower in the landraces than in B73 both under control conditions and after UV-B exposure, suggesting that smRNAs regulate P1 expression by UV-B in maize leaves. Finally, we investigated if different P1 targets in flower tissues are also regulated by this transcription factor in response to UV-B. Some targets analyzed show an induction in maize landraces in response to UV-B, with higher basal expression levels in the landraces than in B73; however, not all the transcripts analyzed were found to be regulated by UV-B in leaves.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 34 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 26%
Researcher 6 18%
Professor 4 12%
Student > Doctoral Student 2 6%
Lecturer 1 3%
Other 2 6%
Unknown 10 29%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 41%
Biochemistry, Genetics and Molecular Biology 4 12%
Nursing and Health Professions 1 3%
Computer Science 1 3%
Chemistry 1 3%
Other 1 3%
Unknown 12 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 May 2016.
All research outputs
#18,453,763
of 22,865,319 outputs
Outputs from Frontiers in Plant Science
#13,794
of 20,233 outputs
Outputs of similar age
#219,232
of 299,499 outputs
Outputs of similar age from Frontiers in Plant Science
#283
of 489 outputs
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