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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Optimization of Agroinfiltration in Pisum sativum Provides a New Tool for Studying the Salivary Protein Functions in the Pea Aphid Complex
|
|---|---|
| Published in |
Frontiers in Plant Science, August 2016
|
| DOI | 10.3389/fpls.2016.01171 |
| Pubmed ID | |
| Authors |
Endrick Guy, Hélène Boulain, Yoann Aigu, Charlotte Le Pennec, Khaoula Chawki, Stéphanie Morlière, Kristina Schädel, Grit Kunert, Jean-Christophe Simon, Akiko Sugio |
| Abstract |
Aphids are piercing-sucking insect pests and feed on phloem sap. During feeding, aphids inject a battery of salivary proteins into host plant. Some of these proteins function like effectors of microbial pathogens and influence the outcome of plant-aphid interactions. The pea aphid (Acyrthosiphon pisum) is the model aphid and encompasses multiple biotypes each specialized to one or a few legume species, providing an opportunity to investigate the underlying mechanisms of the compatibility between plants and aphid biotypes. We aim to identify the aphid factors that determine the compatibility with host plants, hence involved in the host plant specialization process, and hypothesize that salivary proteins are one of those factors. Agrobacterium-mediated transient gene expression is a powerful tool to perform functional analyses of effector (salivary) proteins in plants. However, the tool was not established for the legume species that A. pisum feeds on. Thus, we decided to optimize the method for legume plants to facilitate the functional analyses of A. pisum salivary proteins. We screened a range of cultivars of pea (Pisum sativum) and alfalfa (Medicago sativa). None of the M. sativa cultivars was suitable for agroinfiltration under the tested conditions; however, we established a protocol for efficient transient gene expression in two cultivars of P. sativum, ZP1109 and ZP1130, using A. tumefaciens AGL-1 strain and the pEAQ-HT-DEST1 vector. We confirmed that the genes are expressed from 3 to 10 days post-infiltration and that aphid lines of the pea adapted biotype fed and reproduced on these two cultivars while lines of alfalfa and clover biotypes did not. Thus, the pea biotype recognizes these two cultivars as typical pea plants. By using a combination of ZP1109 and an A. pisum line, we defined an agroinfiltration procedure to examine the effect of in planta expression of selected salivary proteins on A. pisum fitness and demonstrated that transient expression of one candidate salivary gene increased the fecundity of the aphids. This result confirms that the agroinfiltration can be used to perform functional analyses of salivary proteins in P. sativum and consequently to study the molecular mechanisms underlying host specialization in the pea aphid complex. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 50% |
| Switzerland | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 49 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 11 | 22% |
| Researcher | 10 | 20% |
| Student > Bachelor | 4 | 8% |
| Other | 3 | 6% |
| Student > Doctoral Student | 2 | 4% |
| Other | 4 | 8% |
| Unknown | 15 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 45% |
| Biochemistry, Genetics and Molecular Biology | 10 | 20% |
| Environmental Science | 2 | 4% |
| Nursing and Health Professions | 1 | 2% |
| Medicine and Dentistry | 1 | 2% |
| Other | 0 | 0% |
| Unknown | 13 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 August 2016.
All research outputs
#18,467,278
of 22,882,389 outputs
Outputs from Frontiers in Plant Science
#13,816
of 20,270 outputs
Outputs of similar age
#279,252
of 361,775 outputs
Outputs of similar age from Frontiers in Plant Science
#292
of 464 outputs
Altmetric has tracked 22,882,389 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,270 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
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